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Acetylcholinesterase (AchE) Activity Assay Kit

Cat.No.:E-BC-K174-M
Detection instrument: Microplate reader(optimum wavelength: 412 nm)

Detection method: Colorimetric method

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Detection principle

AchE catalyzes the hydrolysis of acetylcholine to form choline, and choline react with dithio p-nitrobenzoic acid (DTNB) to form 5-mercapto-nitrobenzoic acid (TNB). TNB has an absorption peak at 412nm. And the activity of AchE is calculated by measuring the increasing rate of absorbance at 412nm.

Performance characteristics

Synonyms	AchE
Sample type	Serum,plasma,animal tissue and cell
Sensitivity	1.225 U/mL
Detection range	1.225-490 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	20 min
Precision	Average inter-assay CV: 9.300%Average intra-assay CV: 4.700%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

The activity of AchE was significantly decreased comparing with control groups. (P<0.05 versus age-matched control)

A F Odetayo et al investigate the effect of bisphenol F on sexual performance and quality. Acetylcholinesterase (AchE) activity of rat plasma was determined using AchE activity assay kit (E-BC-K174-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (13.88-320.13 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Mouse serum	8-20
Mouse plasma	4-10
Human serum	4-10
Human plasma	4-10
Rat serum	4-10
Dog serum	4-10
Horse serum	2-8
10% Mouse liver tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Mouse brain tissue homogenate	2-8
10% Crucian carp muscle tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Metabolites (2022) IF: 5.581
The Protective and Therapeutic Anti-Alzheimer Potential of Olea europaea L. cv. Picual: An In Silico and In Vivo Study

DOI: 10.3390/metabo12121178

Sample: serum,brain
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

DOI: 10.1016/j.jep.2022.115306

PMID: 35443217
Sample: Brain
Scientific Reports (2021) IF: 4.38
Cacao powder supplementation attenuates oxidative stress, cholinergic impairment, and apoptosis in d-galactose-induced aging rat brain

DOI: 10.1038/s41598-021-96800-y

PMID: 34504131
Sample: brain
HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
Cell membrane enveloped polymeric nanosponge for detoxification of chlorpyrifos poison: In vitro and in vivo studies:

DOI: 10.1177/0960327121993207

Sample: RBC ghosts（红细胞）
Veterinary Research Forum (2021) IF: 1.054
Ethephon causes reproductive malfunction in adult male mice: Histological and biochemical evidence

DOI: 10.30466/vrf.2020.109762.2607

Sample: serum,erythrocyte
Heliyon (2021)
Apoptotic inducement of neuronal cells by codeine: possible role of disrupted redox state and caspase 3 signaling

DOI: 10.1016/j.heliyon.2021.e07481

Sample: brain

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