Acid Phosphatase (ACP) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K094-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $200
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(520 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.

      Performance characteristics

      Synonyms ACP
      Sample type serum,plasma,urine,tissue,cells
      Sensitivity 0.27 U/100 mL
      Detection range 0.27-40 U/100 mL
      Precision Average inter-assay CV: 7.1%Average intra-assay CV: 2.8%
      Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 3 months
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      MSDS for ELISA ISO 9001

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