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Acid Phosphatase (ACP) Activity Assay Kit

Cat.No.:E-BC-K094-S
Detection instrument: Spectrophotometer(520 nm)

Detection method: Colorimetric method

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Detection principle

Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.

Performance characteristics

Synonyms	ACP
Sample type	Serum,plasma,urine,tissue,cells
Sensitivity	0.27 U/100 mL
Detection range	0.27-40 U/100 mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Precision	Average inter-assay CV: 7.100%Average intra-assay CV: 2.800%
Other instruments required	Micropipettor, Incubator, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.27-40 U/100 mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human milk	1
Human saliva	1
Human urine	1
Human serum	1
HepG2 cells homogenization	2-8
10% Mouse kidney tissue homogenization	8-12
10% Mouse liver tissue homogenization	8-12
10% Mouse spleen tissue homogenization	8-12

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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