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Acid Phosphatase (ACP) Activity Assay Kit(PNPP Method)

Cat.No.:E-BC-K010-M
Detection instrument: Microplate reader (400-415 nm, optimum wavelength: 405 nm)

Detection method: Colorimetric method

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96T
500 Assays

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Detection principle

Disodium p-nitrobenzene phosphate (p-NPP), a widely used phosphatase chromogenic substrate, can form p- nitrophenol under the action of acid phosphatase. Under alkaline conditions, p-nitrophenol is yellow and has a maximum absorption peak at 405 nm. The darker of the yellow product is, the higher of the ACP activity is. Therefore, the activity of ACP can be calculated by measuring the OD value at 405 nm.

Performance characteristics

Synonyms	ACP
Sample type	Serum, plasma, tissue
Sensitivity	0.2 U/L
Detection range	0.2–50 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 4.500%Average intra-assay CV: 2.800%
Other instruments required	Micropipettor, 37℃ Incubator
Other reagents required	PBS (0.01 M, pH 7.4)
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.2–50 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Epipremnum aureum tissue homogenate	5-10
Mouse plasma	5-10
Rat plasma	5-10
Human urine	1
Human plasma	5-10
10% Rat spleen tissue homogenate	20-30
10% Rat liver tissue homogenate	20-30
10% Rat kidney tissue homogenate	20-30

Note: The diluent is PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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