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ACTA2 Monoclonal Antibody

Cat:E-AB-22155
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Hela,3T3,Rat brain
Verified Samples in IHC:Mouse cecal
Dilution

WB 1:5000-50000, IHC 1:1000-2000

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Synthetic Peptide
Abbre ACTA2
Synonyms a actin;AAT6;ACTA;ACTA2;Actin alpha 2 smooth muscle aorta;Actin aortic smooth muscle;Actin;aortic smooth muscle;ACTSA;ACTVS;Alpha 2 actin;Alpha actin 2;Alpha cardiac actin;alpha sma;Alpha-actin-2;Cell growth inhibiting gene 46 protein;Cell growth-inhibiting gene 46 protein;GIG46;Growth inhibiting gene 46;MYMY5
Swissprot
Observed MW 42kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm>cytoskeleton.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Purification Method Protein A purification
Research Areas Cancer; Cardiovascular; Developmental Biology; Signal Transduction; Stem Cells
Clone No. 1E12
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.