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Adenosine Triphosphate (ATP) Chemiluminescence Assay Kit

  • Cat.No.:E-BC-F002

  • Detection instrument: Chemiluminescence detector

To Purchase E-BC-F002

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Price: $450

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Detection principle

Under the catalyzation of luciferase, ATP react with luciferin and emits fluorescence, and the fluorescence intensity is proportional to the concentration of ATP within a certain range.

Performance characteristics

Synonyms ATP
Sample type Animal tissue, Cells
Sensitivity 0.003 μmol/L
Detection range 0.003-10 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 15 min
Precision Average inter-assay CV: 6.5%Average intra-assay CV: 2.2%
Other instruments required Vortex mixer, Centrifuge
Storage -20℃
Valid period 3 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.003-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Mouse heart tissue homogenate


10% Mouse kidney tissue homogenate


10% Mouse muscle tissue homogenate


10% Mouse liver tissue homogenate


10% Mouse brain tissue homogenate


10% Mouse lung tissue homogenate


Note: The diluent is reagent 1.

Ralated product

Cat Number Product Name
E-BC-K157-S Adenosine Triphosphate (ATP) Colorimetric Assay Kit


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