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Adenosine Triphosphate (ATP) Chemiluminescence Assay Kit

  • Cat.No.:E-BC-F002

  • Detection instrument: Chemiluminescence detector

To Purchase E-BC-F002

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  • 96T
  • 48T
Price: $450
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Detection principle

Under the catalyzation of luciferase, ATP react with luciferin and emits fluorescence, and the fluorescence intensity is proportional to the concentration of ATP within a certain range.

Performance characteristics

Synonyms ATP
Sample type Animal tissue, Cells
Sensitivity 0.003 μmol/L
Detection range 0.003-10 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 15 min
Precision Average inter-assay CV: 6.5%Average intra-assay CV: 2.2%
Other instruments required Vortex mixer, Centrifuge
Storage -20℃
Valid period 3 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.003-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Mouse heart tissue homogenate

1

10% Mouse kidney tissue homogenate

1

10% Mouse muscle tissue homogenate

1

10% Mouse liver tissue homogenate

1

10% Mouse brain tissue homogenate

1

10% Mouse lung tissue homogenate

1

Note: The diluent is reagent 1.

Ralated product

Cat Number Product Name
E-BC-K157-S Adenosine Triphosphate (ATP) Colorimetric Assay Kit

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

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