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Elab Fluor® 647 Anti-Mouse Perforin Antibody[S16009A]

Uniprot : P10820
  • Cat.No.:E-AB-F1294M

  • Applications: ICFCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

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Antigen Details

Alternate Names Prf1, Pfp, Pfn, Pfn-1,Cytolysin,Lymphocyte pore-forming protein
Swissprot P10820
Gene ID 18646 
References
1.Podack ER, et al. 1988. Immunol. Rev. 103:203.
2.Konjar S, et al. 2010. Immunology. 131:257.
3.Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735.
4.Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.

Product Details

Form Liquid
Clone No. S16009A(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control Elab Fluor® 647 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832M]
Reactivity Mouse
Application ICFCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Elab Fluor® 647
Elab Fluor® 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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