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AF/LE Purified Anti-Mouse CD103 Antibody[M290] E-AB-F10900

Uniprot : Q60677
  • Cat.No.:E-AB-F10900

  • Reactivity: Mouse

To Purchase E-AB-F10900

Size:
  • 50μg
  • 500μg
  • 1mg
Price: $75
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Antigen Details

Alternate Names Integrin alpha-E,Itgae,Integrin alpha M290,CD103
Swissprot Q60677
References
1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Product Details

Form Liquid
Clone No. M290(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control AF/LE Purified Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F098330]
Reactivity Mouse
Application FCM,Block (See the Application Description)
Storage Buffer 0.2 μm filtered in PBS, pH7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or preservatives. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 μg per 106 cells in 100 μL volume or 100 μL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation None (Purified antibody/Azide Free/Low endotoxin)

Background

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F10900 AF/LE Purified Anti-Mouse CD103 Antibody[M290] E-AB-F10900 50μg, 500μg, 1mg
E-AB-F1090A Purified Anti-Mouse CD103 Antibody[M290] E-AB-F1090A 50μg, 100μg
E-AB-F1090B Biotin Anti-Mouse CD103 Antibody[M290] E-AB-F1090B 50μg, 100μg
E-AB-F1090C FITC Anti-Mouse CD103 Antibody[M290] E-AB-F1090C 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1090D PE Anti-Mouse CD103 Antibody[M290] E-AB-F1090D 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1090E APC Anti-Mouse CD103 Antibody[M290] E-AB-F1090E 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1090F
E-AB-F1090G PE/Cyanine5 Anti-Mouse CD103 Antibody[M290] E-AB-F1090G 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1090H PE/Cyanine7 Anti-Mouse CD103 Antibody[M290] E-AB-F1090H 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1090I PE/Cyanine5.5 Anti-Mouse CD103 Antibody[M290] E-AB-F1090I 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1090J
E-AB-F1090L AF488 Anti-Mouse CD103 Antibody[M290] 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1090M AF647 Anti-Mouse CD103 Antibody[M290] E-AB-F1090M 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1090UC FITC Anti-Mouse CD103 Antibody[M290] E-AB-F1090UC 25μg, 50μg, 100μg, 200μg
E-AB-F1090UD PE Anti-Mouse CD103 Antibody[M290] E-AB-F1090UD 25μg, 50μg, 100μg, 200μg
E-AB-F1090UE APC Anti-Mouse CD103 Antibody[M290] E-AB-F1090UE 25μg, 50μg, 100μg, 200μg
E-AB-F1090UF
E-AB-F1090UG PE/Cyanine5 Anti-Mouse CD103 Antibody[M290] E-AB-F1090UG 25μg, 50μg, 100μg, 200μg
E-AB-F1090UH PE/Cyanine7 Anti-Mouse CD103 Antibody[M290] E-AB-F1090UH 25μg, 50μg, 100μg, 200μg
E-AB-F1090UI PE/Cyanine5.5 Anti-Mouse CD103 Antibody[M290] E-AB-F1090UI 25μg, 50μg, 100μg, 200μg
E-AB-F1090UJ
E-AB-F1090UL AF488 Anti-Mouse CD103 Antibody[M290] E-AB-F1090UL 25μg, 50μg, 100μg
E-AB-F1090UM AF647 Anti-Mouse CD103 Antibody[M290] E-AB-F1090UM 25μg, 50μg, 100μg, 200μg

Citations

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