Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD1d Antibody[19G11] E-AB-F10320
Cat.No.:E-AB-F10320
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Alternate Names | Antigen-presenting glycoprotein CD1d1,Cd1d1,CD1d.1,Cd1d1,Cd1.1 |
Swissprot | P11609 |
References |
1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663. 3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535. 4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241. |
Form | Liquid |
Clone No. | 19G11(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD1d is a type I transmembrane protein and member of the MHC family, with a molecular weight ranging from 43-49 kD, depending on the glycosylation degree. CD1d is expressed by antigen presenting cells such as dendritic cells, monocytes, macrophages and B cells; also expressed by thymocytes and intestinal epithelial cells. CD1d present glycolipids to iNKT cells that recognize them by their Vα14i TCR. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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