Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]
Cat.No.:E-AB-F11320
Welcome to order from local distributors.
FAQ for Flow Cytometry Antibody
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Alternate Names | B7-H1, PD-L1,Programmed cell death ligand 1,B7 homolog 1,B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1 |
Swissprot | Q9EP73 |
References |
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365. 3. Freeman G, et al. 2000. J. Exp. Med. 192:1027. |
Form | Liquid |
Clone No. | 10F.9G2(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430] |
Reactivity | Mouse |
Application | FCM,Block (See the Application Description) |
Storage Buffer | 0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | None (Purified antibody-Azide Free/Low endotoxin) |
How to select the appropriate detection channel through the spectrogram?
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
Biotin Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 488 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 647 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 50Tests, 100Tests, 100Tests×2 | Check More |
FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
PE Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
Elab Fluor® 488 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
Elab Fluor® 647 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2] | 25μg, 100μg | Check More |
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!