Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> AF/LE Purified Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]

AF/LE Purified Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]

Uniprot : P35461,P0CW03

Cat.No.:E-AB-F11200
Applications: Depletion,FCM
Isotype: Rat IgG2b, κ
Host: Rat

Applications:

Depletion,FCM

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1mg

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Antigen Details

Alternate Names	Gr-1,Gr1,Ly-6G/Ly-6C,Ly6G/Ly6C
Swissprot	P35461,P0CW03
References	1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. 2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. 3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Product Details

Form	Liquid
Clone No.	RB6-8C5(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	AF/LE Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F098430]
Reactivity	Mouse
Application	Depletion,FCM (See the Application Description)
Storage Buffer	0.2 μm filtered in PBS, pH 7.2. Azide Free (AF)/Low Endotoxin (LE): Contains no stabilizers or stabilizers. Endotoxin level is < 2 EU/mg as Determined by LAL gel clotting assay.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.1 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	None (Purified antibody-Azide Free/Low endotoxin)

How to select the appropriate detection channel through the spectrogram?

Background

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
Biotin Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
FITC Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
PE Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
PerCP Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]	25μg, 100μg	Check More

Citations

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