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Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)

Name: Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method)
Brand: Elabscience
SKU: E-BC-K057-M
Price: 150 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(7)

Cat.No.:E-BC-K057-M
Detection instrument: Microplate reader(620-640 nm)

Detection method: Colorimetric method

Manual MSDS

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48T

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Protocols for metabolism assays

FAQ for metabolism assays

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Detection principle

Bromocresol green (BCG) is widely used as protein staining agent. BCG can combine the albumin in pH 4.0~4.2 to form an albumin-BCG complex. And the color changed from yellow to green. The depth of color is proportional to the concentration of albumin. The content of albumin in serum can be calculated indirectly by measuring the OD value at 630 nm.

Performance characteristics

Synonyms	ALB
Sample type	Serum,plasma
Sensitivity	0.08 g/L
Detection range	0.08-15 g/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 4.600%Average intra-assay CV: 1.500%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	Reagent 1: 2-8℃, Reagent 2: -20℃
Valid period	12 months

Images

ALB level was significantly induced in STZ treated group.

A A-R Mohamed et al found that selenium nanoparticle can alleviate cardio-hepatic damage in type II diabetes. Albumin (ALB) level in rat serum was determined using ALB colorimetric assay kit (E-BC-K057-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.08-15 g/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	8-15
Human plasma	8-15
HepG2 supernatant	1
Mouse plasma	8-15
Rat serum	8-15

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Ameliorative Effects of a Rhenium (V) Compound with Uracil-Derived Ligand Markers Associated with Hyperglycaemia-Induced Renal Dysfunction in Diet-Induced Prediabetic Rats

DOI: 10.3390/ijms232315400

Sample: plasma,urine
American Journal of Translational Research (2021) IF: 4.06
Personalized nursing improves physical condition and life quality of patients undergoing interventional therapy for liver cancer

PMID: 35035768
Sample: Blood
Drug Design Development and Therapy (2021) IF: 4.162
Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

DOI: 10.2147/DDDT.S326975

PMID: 34703210
Sample: serum
Frontiers in Physiology (2019) IF: 3.201
Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides

DOI: 10.3389/fphys.2019.01430

PMID: 31824338
Sample: Plasma
International Journal of Environmental Research and Public Health (2022) IF: 3.39
Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

DOI: 10.3390/ijerph19010498

PMID: 35010758
Sample: plasma
Journal of Oleo Science (2022) IF: 1.628
Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

DOI: 10.5650/jos.ess22033

PMID: 35965085
Sample: serum
PERITONEAL DIALYSIS INTERNATIONAL (2021) IF: 1.756
Hyaluronan reduces colitis-induced intraperitoneal inflammation during peritoneal dialysis:

DOI: 10.1177/08968608211014568

Sample: Serum

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Verified Customer

a******eSubmitted [ Jun 05 2019 ]

Application:Biochemical
Description:When I first saw this kit, I was attracted by the time required by the whole experiment process, and bought it with the mentality of trying it out. The result was in line with my expectation,but,It\'s a little tricky to do the standard curve，i still want to have a single standard to do the calculation.

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J***********kSubmitted [ Feb 12 2021 ]

Asked: Hello, I am looking into developing a simple positive identification in my sample. I basically need an assay that will show if albumin is present or absent, I don\'t need to find the concentration as long as their a big difference in the OD values between the (background) and the reference standard. Do you think that the Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method) fits the job? if not, what would you reccommend

adminSubmitted [ Feb 12 2021 ]

Answered: Not at all.

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