Alkaline Phosphatase (ALP) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K091-S

      • 100Assays
      • 50Assays
      - +
      Price: $200
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(520 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Detection principle

      Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

      Performance characteristics

      Synonyms ALP
      Sample type serum,plasma,urine,tissue,cells
      Sensitivity 0.2 King unit/100 mL
      Detection range 0.2-55.6 King unit/1
      Precision Average inter-assay CV: 5.6%Average intra-assay CV: 2.1%
      Other instruments required Micropipettor, Incubator, Vortex mixer, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months


      1. Isopsoralen regulates PPAR‑γ/WNT to inhibit oxidative stress in osteoporosis
        IF: 1.851
        Journal: Molecular Medicine Reports(2018)
        DOI: 10.3892/mmr.2017.7954
        PMID: 29115612
        Products: E-BC-K091-S 
        Sample type: Serum
        Reactivity: Rat
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      MSDS for ELISA ISO 9001

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