Alkaline Phosphatase (ALP) Activity Assay Kit (E-BC-K091-S)
For research use only.
| Detection Principle | Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value. |
| Synonyms | ALP |
| Sample Type | Serum,Plasma,Urine,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (520 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.2 King unit/100 mL |
| Detection Range | 0.2-55.6 King unit/1 |
| Precision | inter-assay CV: 5.6% | intra-assay CV: 2.1% |
| Sample Volume | 50 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human urine | 1 |
| Rat serum | 1 |
| Cellular supernatant | 1 |
| 10% Mouse kidney tissue homogenization | 30-50 |
| 10% Mouse liver tissue homogenization | 1 |
| HePG2 cell | 1 |
| 10% Mouse brain tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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