Alkaline Phosphatase (ALP) Activity Assay Kit

    • Biochemical Assay Kit-Elabscience
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    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K091-M

      Size:
      • 96T
      • 500Assays
      Qty:
      - +
      Price: $150
      Can detect 80 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(500-530 nm,optimum wavelength: 520 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.

      Performance characteristics

      Synonyms ALP
      Sample type Serum,plasma,tissue,cells
      Sensitivity 0.13 King unit/100 m
      Detection range 0.13-50 King unit/10
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 30 min
      Precision Average inter-assay CV: 8.5%Average intra-assay CV: 5.1%
      Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.13-50 King unit/100 mL).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human plasma

      1

      Human urine

      1

      Rat serum

      1

      Cells culture supernatant

      1

      10% Mouse kidney tissue homogenate

      30-50

      0% Mouse liver tissue homogenate

      1

      10% Mouse brain tissue homogenate

      1

      HepG2 cells

      1

      Note: The diluent is normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4).

      Citations

      1. Omega-3 fatty acids moderate oxidative and proinflammatory events in experimental hepatotoxicity in Wistar rats: comparison with livolin
        Journal: Synergy(2018)
        DOI: 10.1016/j.synres.2018.08.001
        PMID: 99999999
        Products: E-BC-K025-M E-BC-K031-M E-BC-K091-M E-BC-K096-M E-BC-K210 E-BC-K235-M E-BC-K236-M E-EL-0076 
        Sample type: Plasma
        Reactivity: Rat
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      MSDS for ELISA ISO 9001

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