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α-Amylase Activity Assay Kit

Cat.No.:E-BC-K007-M
Detection instrument: Microplate reader(540 nm)

Detection method: Colorimetric method

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96T
48T

Price: $150

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Protocols for metabolism assays

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Detection principle

The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance, which is inactivated by the thermolabile nature of β-amylase, and then the enzyme activity of α-amylase is determined.

Performance characteristics

Sample type	serum, saliva, animal and plant tissue
Sensitivity	0.0097 U/mL
Detection range	0.0097-0.3474 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	40 min
Precision	Average inter-assay CV: 3.900%Average intra-assay CV: 3%
Other instruments required	Micropipettor, Multi-channel pipettor, Incubator, Vortex mixer, Centrifuge
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.97-34.74 U/g tissue).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
1% Epipremnum aureum tissue homogenate	1
1% Green pepper tissue homogenate	1
1% Corn grain tissue homogenate	1
1% Daucus carota tissue homogenate	1

Note: The diluent is double distilled water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

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Reviews/Q&A

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Reviews
Q&A

R*********aSubmitted [ Mar 01 2024 ]

Asked: What is the concentration of the enzyme (U/mL) ?

adminSubmitted [ Mar 01 2024 ]

Answered: This kit can measure α-amylase activity in serum, saliva, animal and plant tissue samples. Sensitivity: 0.0097 U/mL

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