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α-Amylase and β-amylase Activity Assay Kit

Cat.No.:E-BC-K006-M
Detection instrument: Microplate reader(535 nm-540 nm,optimum wavelength: 540 nm)

Detection method: Colorimetric method

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96T

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Detection principle

The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. Amylase activity can be calculated by measuring the OD value at 540 nm.

Performance characteristics

Sample type	Serum, plasma, saliva, tissue
Sensitivity	0.008 U/mL
Detection range	0.01-0.56 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	30 min
Precision	Average inter-assay CV: 3.6%Average intra-assay CV: 2.8%
Other instruments required	Test tubes, Vortex Mixer, Centrifuge, Water bath
Storage	2-8℃
Valid period	6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-0.37 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	10-15
Human serum	10-15
Human saliva	15-25
Mouse serum	15-25
Rat serum	15-25
1% Corn grain tissue homogenate	2-5

Note: The diluent is double distilled water.

Ralated product

Cat Number	Product Name
E-BC-K005-M	β-Amylase Activity Assay Kit
E-BC-K007-M	α-Amylase Activity Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

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