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α-Amylase and β-Amylase Activity Assay Kit

Cat:E-BC-K006-M Citations (1)
Manual MSDS

Price: $ 150

Size:
96T
Quantity:
  • Sample Type: serum;plasma;saliva;tissue
  • Detection Instrument: Microplate reader(535 nm-540 nm,optimum wavelength: 540 nm)
  • Reasearch Areas: Amino acids proteins
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For research use only. Order now, ship in 3 days

Product Details
Detection Principle The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. Amylase activity can be calculated by measuring the OD value at 540 nm.
Sample Type serum,plasma,saliva,tissue
Sensitivity 0.008 U/mL
Detection Range 0.01-0.56 U/mL
Detection Method Colorimetric method
Assay Time 30 min
Precision inter-assay CV: 3.6% | intra-assay CV: 2.8%
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human plasma 10-15
Human serum 10-15
Human saliva 15-25
Mouse serum 15-25
Rat serum 15-25
1% Corn grain tissue homogenate 2-5
The diluent is double distilled water. For the dilution of other sample types, please do pretest to confirm the dilution factor.