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α-Amylase and β-amylase Activity Assay Kit

  • Cat.No.:E-BC-K006-M

  • Detection instrument: Microplate reader(535 nm-540 nm,optimum wavelength: 540 nm)

To Purchase E-BC-K006-M

  • 96T
Price: $150

Detection principle

The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. Amylase activity can be calculated by measuring the OD value at 540 nm.

Performance characteristics

Sample type Serum, plasma, saliva, tissue
Sensitivity 0.008 U/mL
Detection range 0.01-0.56 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 30 min
Precision Average inter-assay CV: 3.6%Average intra-assay CV: 2.8%
Other instruments required Test tubes, Vortex Mixer, Centrifuge, Water bath
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-0.37 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human plasma


Human serum


Human saliva


Mouse serum


Rat serum


1% Corn grain tissue homogenate


Note: The diluent is double distilled water.


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