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α-Ketoglutarate Dehydrogenase(α-KGDH) Activity Assay Kit

  • Cat.No.:E-BC-K083-M

  • Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

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Price: $360

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Detection principle

α-ketoglutarate dehydrogenase (α-KGDH) is a key enzyme in the tricarboxylic acid (TCA) cycle. While the substrate being catalyzed by α-KGDH, NAD+ is reduced to NADH, and electrons are transfered to WST-8 under the action of hydrogen transmitter to produce yellow product. The activity of α-KGDH can be calculated by measuring the change of absorbance value at 450 nm

Performance characteristics

Synonyms α-KGDH
Sample type serum, plasma, animal and plant tissue
Sensitivity 8.3 U/L
Detection range 8.3-42.3 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 40 min
Precision Average inter-assay CV: 8.500%Average intra-assay CV: 1.500%
Other instruments required Incubator, Centrifuge
Storage -20℃
Valid period 12 months


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