NADP⁺/NADPH Colorimetric Assay Kit (WST-8) (E-BC-K803-M)
For research use only.
| Detection Principle |
Detect total content of NADP+ and NADPH: Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measure the OD value at 450 nm. Detect NADPH: After treating sample, heat at 60℃ water bath for 30 min. the NADP+ of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm. Detect NADP+ and NADP+/NADPH: The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained of the first two steps as well as the separate content of NADPH. |
| Synonyms | NADP;NADPH |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450 nm) |
| Research Area | Oxidative Stress , Metabolic Diseases , Glycolysis And Lipid Metabolism , Cuproptosis , Disulfidptosis |
| Other Reagents Required | Ultrapure water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.02 μmol/L |
| Detection Range | 0.02-5.0 μmol/L |
| Precision | inter-assay CV: 5.5% | intra-assay CV: 2.1% |
| Sample Volume | 50-200 μL(tissue/cell homogenate) |
| Assay Time | 1 h 10 min |
Note: Data for this module come from published literature that cites this product, displaying other products used together in the same research paper.
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Jurkat cells | 1 |
| Mark cells | 1 |
| HCT116 cells | 1 |
| 293T cells | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| Hela cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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