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Angiotensin Converting Enzyme (ACE1) Activity Assay Kit

Cat.No.:E-BC-K003-S
Detection instrument: Spectrophotometer(340nm)

Detection method: Colorimetric method

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Detection principle

N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG) have the maximum absorption peak at 340 nm, angiotensin converting enzyme catalyze N-(3[2-Furyl ]Acryloyl)-Phe-Gly-Gly to produce FAP and GG, and the absorbance at 340 nm will be decreased. The activity of ACE1 can be calculated indirectly by measuring the decrease in absorbance at 340 nm.

Performance characteristics

Synonyms	ACE1
Sample type	Serum, plasma, animal tissue
Sensitivity	27.5 U/L
Detection range	27.5- 682 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	10-90 min
Precision	Average inter-assay CV: 4.400%Average intra-assay CV: 3%
Other instruments required	Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required	PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (27.5- 682 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
Rat plasma	1
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat lung tissue homogenate	3-5

Note: The diluent is normal saline.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022) IF: 8.025
Major royal jelly proteins elicited suppression of SARS-CoV-2 entry and replication with halting lung injury

DOI: 10.1016/j.ijbiomac.2022.12.251

Sample: LTC-A549 cells
CHEMICO-BIOLOGICAL INTERACTIONS (2022) IF: 5.168
Modulation of the miR-122/Sirt-6/ACE2 axis on experimentally-induced myocardial infarction

DOI: 10.1016/j.cbi.2022.110276

Sample: heart

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