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APC Anti-Human CD194/CCR4 Antibody[L291H4]

Uniprot : P51679
  • Cat.No.:E-AB-F1366E

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1366E

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $110
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Antigen Details

Alternate Names CKR4, K5-5, CMKBR4, ChemR13, CC-CKR-4, MGC88293, HGCN:14099
Swissprot P51679
Gene ID 1233
References
1. Katschke KJ, et al. 2001 Arthritis Rheum. 44:1022.
2. Colantonio L, et al. 2002 Eur. J. Immunol. 32:1264.
3. Jakubzick C et al. 2004 Am. J. Pathol. 165:1211.
4. Morimoto Y, et al. 2005 J. Leukoc. Biol. 78:753.
5. Baatar J, et al. 2007 J. Immunol. 178:4891.
6. Kusumoto M, et al. 2007 J. Interferon. Cytokine Res. 27:901.

Product Details

Form Liquid
Clone No. L291H4(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD194, also known as CCR4, is a CC chemokine receptor. It binds CCL17 and CCL22 and is expressed on a subset of T and B cells, basophils, monocytes, and NK cells. Human Th2 cells are characterized by the expression of CCR4 and CCR8, and these receptors are regulated differently during Th2 development. Human peripheral blood Tregs can be divided into two distinct populations based on the expression of CCR4. Freshly isolated Tregs express CCR4 and presumably represent memory-type Tregs, and CCR4- Tregs require CD3-mediated activation to acquire a regulatory activity. Depletion of CCR4+ T cells leads to Th1-type polarization of CD4+ T cells and augmentation of CD8+ T cell responses to tumor antigens. CCR4 and its ligands are important for the recruitment of memory T cells into the skin in various cutaneous immune diseases.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
PE Anti-Human CD194/CCR4 Antibody[L291H4] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human CD194/CCR4 Antibody[L291H4] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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