APC Anti-Human CD20 Antibody[2H7] E-AB-F1212E

Uniprot : P11836
  • Host: Mouse
  • Applications: FCM
  • Isotype: Mouse IgG2b, κ

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Antigen Details

Alternate Names Bp35,Leukocyte surface antigen Leu-16,MS4A1,B-lymphocyte surface antigen B1
Swissprot P11836
Gene ID 931
References
1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.

Product Details

Form Liquid
Clone No. 2H7(See Other Available Formats)
Host Mouse
Isotype Mouse IgG2b, κ
Isotype Control APC Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812E]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer PBS with 0.05% Proclin300, 1% BSA
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC

Background

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

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