Click here to view cell apoptosis assay products
×

APC Anti-Mouse Foxp3 Antibody[3G3]

  • Cat.No.:E-AB-F1238E

  • Applications: ICFCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1238E

Size:
  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $174
Qty:

Antigen Details

Alternate Names Forkhead box protein P3,IPEX,JM2,Foxp3
Swissprot Q99JB6
References
1. Hori S, et al. 2003. Science 299:1057.

Product Details

Form Liquid
Clone No. 3G3(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control APC Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792E]
Reactivity Mouse
Application ICFCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation APC
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers (such as CD4 and CD25). Transduced expression of FOXP3 in CD4+/CD25-cells has been shown to induce GITR, CD103, and CTLA4 and impart a T regulatory cell phenotype. FOXP3 is mutated in X-linked autoimmunity-allergic dysregulation syndrome (XLAAD or IPEX) in humans and in 'scurfy' mice. Overexpression of FOXP3 has been shown to lead to a hypoactive immune state suggesting that this transcriptional factor is a central regulator of T cell activity. In human, unlike in mouse, two isoforms of FOXP3 have been reported: one (FOXP3) corresponding to the canonical full-length sequence; the other (FOXP3 δ2) lacking exon 2.

Protocols & Troubleshootings

Related Protocols Staining Intracellular Antigens for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Mouse Foxp3 Antibody[3G3] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse Foxp3 Antibody[3G3] 50Tests, 100Tests, 100Tests×2 Check More

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Oncology Letters (2019) IF: 1.871
    Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome

    DOI: 10.3892/ol.2019.11114

    Sample: Cell lysate

Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for 10 Test Free Trial FCM Antibody
*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?

*Captcha: