APEX1 Polyclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
Size:
200μL
120μL
60μL
20μL
Quantity:
- Host: Rabbit
- Reactivity: Human;Mouse;Rat
- Applications: WB
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Product Details
Verified Samples |
Verified Samples in WB:Hela,Human liver cancer,Raji,Jurkat,293T,PC-3 |
Dilution |
WB 1:500-1:2000 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Recombinant protein of human APEX1 |
Abbre | APEX1 |
Synonyms | AP endonuclease 1;AP endonuclease class I;AP lyase;APE 1;APE;APE-1;APEN;APEX 1;APEX;APEX nuclease (multifunctional DNA repair enzyme) 1;Apex nuclease 1;APEX nuclease;APEX1;APEX1;Apurinic endonuclease;Apurinic-apyrimidinic endonuclease 1;Apurinic/apyrimidinic (abasic) endonuclease;Apurinic/apyrimidinic endonuclease 1;Apurinic/apyrimidinic exonuclease;APX;BAP1;Deoxyribonuclease (apurinic or apyrimidinic);DNA (apurinic or apyrimidinic site) lyase;DNA-(apurinic or apyrimidinic site) lyase;mitochondrial;EC 4.2.99.18;HAP 1;HAP1;Human Apurinic endonuclease 1;MGC139790;Multifunctional DNA repair enzyme;Redox factor 1;Redox factor-1;REF 1;REF 1 protein;REF-1;REF1;REF1 protein |
Swissprot | |
Calculated MW | 36kDa |
Cellular Localization | Mitochondrion. The cleaved APEX2 is only detected in mitochondria (By similarity). Translocation from the cytoplasm to the mitochondria is mediated by ROS signaling and cleavage mediated by granzyme A. Tom20-dependent translocated mitochondrial APEX1 level is significantly increased after genotoxic stress and Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm. |
Concentration | 0.2 mg/mL |
Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Cardiovascular; Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Biological ice pack at 4 ℃ |
background | Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. |
Experiment Operation Video
Protocols
• Western Blot Guide
• Immunohistochemistry Guide for Slide-mounted Paraffin Sections
• Immunofluorescence Guide
• Immunohistochemistry Troubleshooting Tips
• Western Blot Troubleshooting Tips
• Immunofluorescence Troubleshooting Tips
• Comparison of Protein Separation Linear Range
• ImmunoPrecipitation Protocol