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ATP Colorimetric Assay Kit

Cat.No.:E-BC-K157-S
Detection instrument: Spectrophotometer(636 nm)

Detection method: Colorimetric method

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Detection principle

Creatine Kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate, then detected by phosphomolybdic acid colorimetry.

Performance characteristics

Synonyms	ATP
Sample type	Tissue
Sensitivity	0.01 mmol/L
Detection range	0.01-1.5 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	70 min
Precision	Average inter-assay CV: 9.300%Average intra-assay CV: 3.600%
Other instruments required	Micropipettor, Incubator, Water bath, Vortex mixer, Centrifuge
Other reagents required	Deionized water, Normal saline (0.9% NaCl)
Storage	Reagent 4:-20℃;Others:2-8℃
Valid period	12 months

Images

ATP level was reduced significantly upon cells with C18 ceramide and restored with FB1. (***P<0.001, ****P<0.0001)

S Arora et al found that miR-495-3p regulates the mitophagy and apoptosis in NSCLC through Sphk1. ATP level in NSCLC was determined using ATP colorimetric assay kit (E-BC-K157-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-1.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Rat muscle tissue homogenate	2-3
10% Rat liver tissue homogenate	2-3
10% Rat brain tissue homogenate	2-3
10% Rat kidney tissue homogenate	2-3
10% Rat lung tissue homogenate	2-3

Note: The diluent is deionized water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

BIOMATERIALS (2022) IF: 15.304
Crafting ɣ-L-Glutamyl-l-Cysteine layered Human Serum Albumin-nanoconstructs for brain targeted delivery of ropinirole to attenuate cerebral ischemia/reperfusion injury via “3A approach”

DOI: 10.1016/j.biomaterials.2022.121805

Sample: brain
FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
miR-495–3p regulates sphingolipid metabolic reprogramming to induce Sphk1/ceramide mediated mitophagy and apoptosis in NSCLC

DOI: 10.1016/j.freeradbiomed.2022.07.001

Sample: A549 cell,H1299 cell
Materials Today Bio (2021) IF: 7.348
Cascade targeting codelivery of ingenol-3-angelate and doxorubicin for enhancing cancer chemoimmunotherapy through synergistic effects in prostate cancer

DOI: 10.1016/j.mtbio.2021.100189
Frontiers in Molecular Biosciences (2022) IF: 6.113
Glucose Deprivation Induced by Acarbose and Oncolytic Newcastle Disease Virus Promote Metabolic Oxidative Stress and Cell Death in a Breast Cancer Model

DOI: 10.3389/fmolb.2022.816510

PMID: 35936786
Sample: mammary adenocarcinoma tissue
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 6.208
Moderating Gut Microbiome/Mitochondrial Axis in Oxazolone Induced Ulcerative Colitis: The Evolving Role of β-Glucan and/or, Aldose Reductase Inhibitor, Fidarestat

DOI: 10.3390/ijms24032711

Sample: intestinal tissue
LIFE SCIENCES (2022) IF: 5.037
miR-16-5p regulates aerobic glycolysis and tumorigenesis of NSCLC cells via LDH-A/lactate/NF-κB signaling

DOI: 10.1016/j.lfs.2022.120722

PMID: 35714705
Sample: A549 cell,H1299 cell
MOLECULAR NEUROBIOLOGY (2021) IF: 5.59
SETD3 Downregulation Mediates PTEN Upregulation-Induced Ischemic Neuronal Death Through Suppression of Actin Polymerization and Mitochondrial Function

DOI: 10.1007/s12035-021-02459-x

PMID: 34218417
Sample: brain
BIOCHIMIE (2022) IF: 4.079
Glucose deprivation using 2-deoxyglucose and acarbose induce metabolic oxidative stress and apoptosis in female mice bearing breast cancer

DOI: 10.1016/j.biochi.2022.01.007

PMID: 35066100
Sample: breast tumor tissue
NEUROTOXICOLOGY (2022) IF: 4.398
Effects of pramipexole on beta-amyloid1–42 memory deficits and evaluation of oxidative stress and mitochondrial function markers in the hippocampus of Wistar rat

DOI: 10.1016/j.neuro.2022.07.006

Sample: hippocampus tissue
REDOX REPORT (2022) IF: 4.412
Modulating gut dysbiosis and mitochondrial dysfunction in oxazolone-induced ulcerative colitis: the restorative effects of β-glucan and/or celastrol

DOI: 10.1080/13510002.2022.2046425

PMID: 35246012
Sample: intestinal epithelial cell
Frontiers in Oncology (2020) IF: 4.848
The Pan-Sirtuin Inhibitor MC2494 Regulates Mitochondrial Function in a Leukemia Cell Line

DOI: 10.3389/fonc.2020.00820

PMID: 32528892
Sample: Cell lysate
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY (2022) IF: 4.966
Antibacterial activity and action mechanism of flavonoids against phytopathogenic bacteria

DOI: 10.1016/j.pestbp.2022.105221
Scientific Reports (2022) IF: 4.996
Calotropis gigantea stem bark extracts inhibit liver cancer induced by diethylnitrosamine

DOI: 10.1038/s41598-022-16321-0

PMID: 35840761
Sample: liver,HepG2 cell
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2023) IF: 3.094
Metabolic Phenotype Intricacies on Altered Glucose Metabolism of Breast Cancer Cells upon Glut-1 Inhibition and Mimic Hypoxia In Vitro

DOI: 10.1007/s12010-023-04373-5

Sample: SKBR-3 breast cancer cell
Experimental and Therapeutic Medicine (2022) IF: 2.447
Salvianolic acid A promotes mitochondrial biogenesis and function via regulating the AMPK/PGC‑1α signaling pathway in HUVECs

DOI: 10.3892/etm.2022.11412

Sample: Primary HUVECs
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540

Sample: hepatic tissue
JOURNAL OF MOLECULAR NEUROSCIENCE (2022) IF: 2.866
Honokiol Reduces Mitochondrial Dysfunction and Inhibits Apoptosis of Nerve Cells in Rats with Traumatic Brain Injury by Activating the Mitochondrial Unfolded Protein Response

DOI: 10.1007/s12031-022-02089-5

Sample: brain tissues
Biochemistry and Biophysics Reports (2022)
Simple neutralization test report: Do probiotics contribute to COVID-19 therapy?

DOI: 10.1016/j.bbrep.2022.101348

PMID: 36120494
Sample: PBMCs

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Reviews/Q&A

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Reviews
Q&A

A********LSubmitted [ Jun 13 2023 ]

Asked: Could you please explain the reaction principle of ATP Colorimetric and L-Lactate colorimetric assay principle

adminSubmitted [ Jun 13 2023 ]

Answered: Hello Ajeesh B L.Thanks for your support.We will reply you by E-mail.

M*****iSubmitted [ Oct 21 2022 ]

Asked: Does the rat brain tissue need to be diluted? What is the reference dilution?

adminSubmitted [ Oct 21 2022 ]

Answered: The reference dilution of rat brain tissue is 2-3 times.

M*****iSubmitted [ Sep 20 2022 ]

Asked: Is this kit suitable for cell sample？

adminSubmitted [ Sep 20 2022 ]

Answered: Unfortunately, this kit is not suitable for cell sample.You can choose E-BC-F002 to detect cell sample.

A********LSubmitted [ Jun 17 2021 ]

Asked: Please provide a Example analysis for Cell samples.

adminSubmitted [ Jun 17 2021 ]

Answered: Take cell for example and carry the assay according to the operation table. The results are as follows: The average OD value of the blank is 0.048, the average OD value of the standard is 0.376, the average OD value of the sample is 0.343, the average OD value of the control is 0.303, and the calculation result is: ATP（mmol/10^9）=0.04/0.327*1/(1/0.3)=0.04mmol/10^9

A********LSubmitted [ Jun 17 2021 ]

Asked: For this ATP colorimetric assay the protein concentration of Samples required or not?

adminSubmitted [ Jun 17 2021 ]

Answered: If you are going to test the Cell line samples, the protein is not needed to be tested, but it needs to account the cell number.

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