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ATP Colorimetric Assay Kit

Cat.No.:E-BC-K157-M
Detection instrument: Microplate reader(630 nm-640 nm,optimum wavelength: 636 nm)

Detection method: Colorimetric method

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Detection principle

Creatine kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate. The content of phosphocreatine was determined by colorimetric method to reflect the content of ATP.

Performance characteristics

Synonyms	ATP
Sample type	Animal tissue
Sensitivity	0.01 mmol/L
Detection range	0.03-1.5 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 5.400%Average intra-assay CV: 5.100%
Other instruments required	Micropipettor, Tubes, Vortex mixer, Incubator, 100℃ Water bath, Centrifuge
Storage	Reagent 4:-20℃;Others:2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-1.5 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Rat muscle tissue homogenate	2-4
10% Rat liver tissue homogenate	2-4
10% Mouse brain tissue homogenate	2-4
10% Rat kidney tissue homogenate	2-4
10% Rat lung tissue homogenate	2-4

Note: The diluent is double distilled water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Materials Today Bio (2021) IF: 7.348
Cascade targeting codelivery of ingenol-3-angelate and doxorubicin for enhancing cancer chemoimmunotherapy through synergistic effects in prostate cancer

DOI: 10.1016/j.mtbio.2021.100189
Cells (2022) IF: 7.666
Autophagy in Rat Müller Glial Cells Is Modulated by the Sirtuin 4/AMPK/mTOR Pathway and Induces Apoptosis under Oxidative Stress

DOI: 10.3390/cells11172645

Sample: rMC-1 cell
Frontiers in Molecular Biosciences (2022) IF: 6.113
Glucose Deprivation Induced by Acarbose and Oncolytic Newcastle Disease Virus Promote Metabolic Oxidative Stress and Cell Death in a Breast Cancer Model

DOI: 10.3389/fmolb.2022.816510

PMID: 35936786
Sample: mammary adenocarcinoma tissue
Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation

DOI: 10.1155/2022/7655142

Sample: nucleus pulposus(NP) cell
LIFE SCIENCES (2022) IF: 5.037
N-acetyl-L-cysteine ameliorates mitochondrial dysfunction in ischemia/reperfusion injury via attenuating Drp-1 mediated mitochondrial autophagy

DOI: 10.1016/j.lfs.2022.120338

PMID: 35065167
Sample: brain tissue
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY (2022) IF: 4.966
Antibacterial activity and action mechanism of flavonoids against phytopathogenic bacteria

DOI: 10.1016/j.pestbp.2022.105221
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2023) IF: 3.094
Metabolic Phenotype Intricacies on Altered Glucose Metabolism of Breast Cancer Cells upon Glut-1 Inhibition and Mimic Hypoxia In Vitro

DOI: 10.1007/s12010-023-04373-5

Sample: SKBR-3 breast cancer cell
PLoS One (2021) IF: 3.24
Calotropis gigantea stem bark extract induced apoptosis related to ROS and ATP production in colon cancer cells

DOI: 10.1371/journal.pone.0254392

PMID: 34343190
Sample: HCT116 cell
Experimental and Therapeutic Medicine (2022) IF: 2.447
Salvianolic acid A promotes mitochondrial biogenesis and function via regulating the AMPK/PGC‑1α signaling pathway in HUVECs

DOI: 10.3892/etm.2022.11412

Sample: Primary HUVECs
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540

Sample: hepatic tissue
JOURNAL OF MOLECULAR NEUROSCIENCE (2022) IF: 2.866
Honokiol Reduces Mitochondrial Dysfunction and Inhibits Apoptosis of Nerve Cells in Rats with Traumatic Brain Injury by Activating the Mitochondrial Unfolded Protein Response

DOI: 10.1007/s12031-022-02089-5

Sample: brain tissues
Cancer Management and Research (2020) IF: 2.886
CircSEC31A Promotes the Malignant Progression of Non-Small Cell Lung Cancer Through Regulating SEC31A Expression via Sponging miR-376a

DOI: 10.2147/CMAR.S280124

PMID: 33204164
Sample: Cell sample
Biochemistry and Biophysics Reports (2022)
Simple neutralization test report: Do probiotics contribute to COVID-19 therapy?

DOI: 10.1016/j.bbrep.2022.101348

PMID: 36120494
Sample: PBMCs

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