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BCA Protein Colorimetric Assay Kit

  • Cat.No.:E-BC-K318-M

  • Detection instrument: Microplate reader(540-590 nm, optimum wavelength: 562 nm)

To Purchase E-BC-K318-M

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  • 500Assays
  • 96T
Price: $150
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Detection principle

Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.

Performance characteristics

Synonyms TP
Sample type Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity 0.0165 mg/mL
Detection range 0.0165-1 mg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 4.5%Average intra-assay CV: 2.2%
Other instruments required Vortex mixer, Micropipettor, Incubator
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage RT
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.0165-1 mg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Mouse brain tissue homogenization

8-12

10% Mouse kidney tissue homogenization

8-12

Human serum

100-200

10% Rat liver tissue homogenization

15-20

10% Mouse heart tissue homogenization

8-12

Rat serum

100-200

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biomolecules (2020) IF: 4.082
    Post-Treatment of Synthetic Polyphenolic 1,3,4 Oxadiazole Compound A3, Attenuated Ischemic Stroke-Induced Neuroinflammation and Neurodegeneration

    DOI: 10.3390/biom10060816

    PMID: 32466476

    Sample: Tissue homogenate
  2. Frontiers in Neuroscience (2020) IF: 3.707
    Potent Natural Antioxidant Carveol Attenuates MCAO-induced Oxidative stress, Neurodegeneration by Regulating the Nrf-2 pathway

    DOI: 10.3389/fnins.2020.00659

    Sample: Tissue homogenate
  3. European Journal of Pharmacology (2019) IF: 3.04
    The antidepressant effects of hesperidin on chronic unpredictable mild stress-induced mice

    DOI: 10.1016/j.ejphar.2019.03.035

    PMID: 30928632

    Sample: Cell culture supernatant,Tissue homogenate
  4. Brazilian Journal of Cardiovascular Surgery (2020) IF: 1.053
    Effect of Shenfu Injection on Isolated Empty Beating Hearts from Miniature Pigs

    DOI: 10.21470/1678-9741-2019-0264

    Sample: Tissue homogenate
  5. Neuroscience Letters (2018) IF: 2.173
    Regular aerobic exercise correlates with reduced anxiety and incresed levels of irisin in brain and white adipose tissue.

    DOI: 10.1016/j.neulet.2018.04.023

    PMID: 29655944

    Sample: Tissue homogenate
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