BCA Protein Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    <
    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K318-M

      Size:
      • 500Assays
      • 96T
      Qty:
      - +
      Price: $200
      Can detect 400 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Microplate reader(540-590 nm, optimum wavelength: 562 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Detection principle

      Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.

      Performance characteristics

      Synonyms TP
      Sample type Serum,plasma,cell culture supernatant,tissue,cells
      Sensitivity 0.0165 mg/mL
      Detection range 0.0165-1 mg/mL
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 40 min
      Precision Average inter-assay CV: 4.5%Average intra-assay CV: 2.2%
      Other instruments required Vortex mixer, Micropipettor, Incubator
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage RT
      Valid period 12 months

      Citations

      1. Post-Treatment of Synthetic Polyphenolic 1,3,4 Oxadiazole Compound A3, Attenuated Ischemic Stroke-Induced Neuroinflammation and Neurodegeneration
        IF: 4.082
        Journal: Biomolecules(2020)
        DOI: 10.3390/biom10060816
        PMID: 32466476
        Products: E-BC-K318-M 
        Sample type: Tissue homogenate
        Reactivity: Rat
      2. Potent Natural Antioxidant Carveol Attenuates MCAO-induced Oxidative stress, Neurodegeneration by Regulating the Nrf-2 pathway
        IF: 3.707
        Journal: Frontiers in Neuroscience(2020)
        DOI: 10.3389/fnins.2020.00659
        PMID: 99999999
        Products: E-BC-K318-M E-EL-R0019 E-EL-R0488 
        Sample type: Tissue homogenate
        Reactivity: Rat
      3. The antidepressant effects of hesperidin on chronic unpredictable mild stress-induced mice
        IF: 3.04
        Journal: European Journal of Pharmacology(2019)
        DOI: 10.1016/j.ejphar.2019.03.035
        PMID: 30928632
        Products: E-BC-K318-M 
        Sample type: Cell culture supernatant,Tissue homogenate
        Reactivity: Mouse
      4. Effect of Shenfu Injection on Isolated Empty Beating Hearts from Miniature Pigs
        IF: 1.053
        Journal: Brazilian Journal of Cardiovascular Surgery(2020)
        DOI: 10.21470/1678-9741-2019-0264
        PMID: 99999999
        Products: E-BC-K318-M 
        Sample type: Tissue homogenate
        Reactivity: Porcine
      5. Assessment of the Effects of Crude Methanolic Extracts (Leaf and Twig) of Loranthus micranthus on Streptozotocin Induced Diabetic Rats
        Journal: Journal of Diabetes and Islet Biology(2020)
        DOI: 10.31579/ 2641-8975/015
        PMID: 99999999
        Products: E-BC-K073-S E-BC-K109-M E-BC-K221 E-BC-K238 E-BC-K318-M E-EL-R0019 E-EL-R3034 
        Sample type: Plasma
        Reactivity: Rat
      6. Regular aerobic exercise correlates with reduced anxiety and incresed levels of irisin in brain and white adipose tissue.
        IF: 2.173
        Journal: Neuroscience Letters(2018)
        DOI: 10.1016/j.neulet.2018.04.023
        PMID: 29655944
        Products: E-BC-K318-M 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      Show AllShorten
      submit review
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA ISO 9001

      Browsing History


        People Also Bought

        Apply for
        *Product Name:
        *Catalog Number:
        *Name:
        *Email:
        *Message:
        *Country:
        How do you know
        Elabscience:

        *Captcha: