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β-Amylase Activity Assay Kit

Cat.No.:E-BC-K005-M
Detection instrument: Microplate reader(535 nm-540 nm,optimum wavelength: 540 nm)

Detection method: Colorimetric method

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96T
500Assays

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Detection principle

The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. β-amylase was inactivated by the property of amylase not to be heat-resistant, and then the enzyme activity of total amylase and α-amylase is determined. So the activity of β-amylase can be calculated indirectly.

Performance characteristics

Sample type	Plant tissue
Sensitivity	0.97 U/g tissue
Detection range	0.97-34.74 U/g tissu
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	40 min
Precision	Average inter-assay CV: 3.200%Average intra-assay CV: 2.300%
Other instruments required	Test tubes, Vortex Mixer, Centrifuge, Water bath
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.97-34.74 U/g tissue).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
1% Epipremnum aureum tissue homogenate	1
1% Green pepper tissue homogenate	1
1% Corn grain tissue homogenate	1
1% Daucus carota tissue homogenate	1

Note: The diluent is double distilled water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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