Alternate Names	Intercellular adhesion molecule 1,Icam1,MALA-2,MyD10,CD54,Icam-1
Swissprot	P13597
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270.

Product Details

Form	Liquid
Clone No.	YN1/1.7.4(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	Biotin Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843B]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Biotin

How to select the appropriate detection channel through the spectrogram?

Background

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD54 Antibody[YN1/1.7.4]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
FITC Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
PE Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
APC Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More

Citations

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