Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Biotin Anti-Mouse CD54 Antibody[YN1/1.7.4]
Cat.No.:E-AB-F1018B
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Alternate Names | Intercellular adhesion molecule 1,Icam1,MALA-2,MyD10,CD54,Icam-1 |
Swissprot | P13597 |
References |
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270. |
Form | Liquid |
Clone No. | YN1/1.7.4(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | Biotin Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843B] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Biotin |
How to select the appropriate detection channel through the spectrogram?
CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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