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Blood Ammonia Colorimetric Assay Kit

Cat.No.:E-BC-K145-S
Detection instrument: Spectrophotometer(635 nm)

Detection method: Colorimetric method

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Detection principle

Blood protein can be precipitated with protein precipitator, and enzyme activity will be destroyed, which can prevent the formation of free ammonia in vitro. Most interfering color substances were removed at the same time, indigo was formed in non-protein filtrate by Berthelot reaction, and the color depth was proportional to the content of blood ammonia. Blood ammonia content can be determined by comparing with standard solution.

Performance characteristics

Synonyms	NH3
Sample type	Serum,plasma
Sensitivity	0.01 mmol/L
Detection range	0.01-2.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	65 min
Precision	Average inter-assay CV: 5%Average intra-assay CV: 4.700%
Other instruments required	Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

NEUROSCIENCE LETTERS (2021) IF: 3.046
Syringic acid protects against thioacetamide-induced hepatic encephalopathy: Behavioral, biochemical, and molecular evidence

DOI: 10.1016/j.neulet.2021.136385

PMID: 34871743
Sample: serum
METABOLIC BRAIN DISEASE (2022) IF: 3.584
Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

DOI: 10.1007/s11011-022-00952-3

PMID: 35298730
Sample: serum
Frontiers in Medicine (2020) IF: 3.9
Helicobacter pylori as an Initiating Factor of Complications in Patients With Cirrhosis: A Single-Center Observational Study

DOI: 10.3389/fmed.2020.00096

Sample: serum

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