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c-Myc Polyclonal Antibody

Cat:E-AB-30975 Citations (3)
Manual MSDS

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Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC-p
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Product Details
Verified Samples Verified Samples in WB:Jurkat
Dilution

WB 1:500-1:2000, IHC 1:100-1:300

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthesized peptide derived from human c-Myc around the non-phosphorylation site of Ser373.
Abbre c-Myc
Synonyms AU016757;Avian myelocytomatosis viral oncogene homolog;bHLHe39;c Myc;Class E basic helix-loop-helix protein 39;MRTL;Myc;Myc protein;Myc proto oncogene protein;Myc proto-oncogene protein;myc-related translation/localization regulatory factor;MYC;Myc2;MYCC;Myelocytomatosis oncogene;Niard;Nird;Oncogene Myc;OTTHUMP00000158589;Proto-oncogene c-Myc;Protooncogene homologous to myelocytomatosis virus;RNCMYC;Transcription factor p64;Transcriptional regulator Myc-A;V-Myc avian myelocytomatosis viral oncogene homolog;v-myc myelocytomatosis viral oncogene homolog (avian)
Swissprot
Calculated MW 49kDa
Observed MW 50-60kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus>nucleoplasm. Nucleus>nucleolus.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer; Epigenetics and Nuclear Signaling; Stem Cells
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.