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Catalase (CAT) Activity Assay Kit

  • Cat.No.:E-BC-K031-S

  • Detection instrument: Spectrophotometer(405 nm)

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Detection principle

The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

Performance characteristics

Synonyms CAT
Sample type Serum,plasma,cells,tissue
Sensitivity 0.27 U/mL
Detection range 0.27-155.4 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 5.100%Average intra-assay CV: 3.100%
Other instruments required Micropipettor, Vortex mixer, Incubator or Water-bath
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.27-155.4 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

The volume of sample

10% Rat liver tissue homogenization

25-50

50 μL

10% Rat kidney tissue homogenization

10-25

50 μL

10% Rat brain tissue homogenization

5-10

50 μL

Human serum

1

100 μL

293T supernatant

1

100 μL

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. JOURNAL OF PINEAL RESEARCH (2022) IF: 13.007
    Melatonin modulates Nrf2 activity to protect porcine pre-pubertal Sertoli cells from the abnormal H2O2 generation and reductive stress effects of cadmium.

    DOI: 10.1111/jpi.12806

    Sample: Sertoli cell (SCs)
  2. Journal of Advanced Research (2022) IF: 10.479
    Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria

    DOI: 10.1016/j.jare.2022.05.010

    PMID: 35640876

    Sample: leaf
  3. JOURNAL OF HAZARDOUS MATERIALS (2021) IF: 10.588
    Sequential use of UV-LEDs irradiation and chlorine to disinfect waterborne fungal spores: Efficiency, mechanism and photoreactivation

    DOI: 10.1016/j.jhazmat.2021.127102

    PMID: 34482083

  4. ACS Applied Materials & Interfaces (2021) IF: 9.2289
    Osmium–Tellurium Nanozymes for Pentamodal Combinatorial Cancer Therapy

    DOI: 10.1021/acsami.1c14201

    Sample: OsTeNRs
  5. Antioxidants (2022) IF: 7.675
    Phenolics and Volatile Compounds of Fennel (Foeniculum vulgare) Seeds and Their Sprouts Prevent Oxidative DNA Damage and Ameliorates CCl4-Induced Hepatotoxicity and Oxidative Stress in Rats

    DOI: 10.3390/antiox11122318

    Sample: serum
  6. Antioxidants (2023) IF: 7.675
    Nephroprotective Effect of Fennel (Foeniculum vulgare) Seeds and Their Sprouts on CCl4-Induced Nephrotoxicity and Oxidative Stress in Rats

    DOI: 10.3390/antiox12020325

    Sample: Serum,kidney tissue
  7. Antioxidants (2022) IF: 7.675
    Inhibition of Tumor Growth and Modulation of Antioxidant Activity of Rhodoxanthin Isolated from Taxus baccata Aril against B16F10 Murine Malignant Melanoma

    DOI: 10.3390/antiox11112264

    Sample: plasma
  8. Frontiers in Molecular Biosciences (2022) IF: 6.113
    Biochemical profiling of metabolomics in heavy metal-intoxicated impaired metabolism and its amelioration using plant-based bioactive compound

    DOI: 10.3389/fmolb.2022.1029729

    PMID: 36330218

    Sample: liver
  9. ACS Applied Nano Materials (2022) IF: 6.14
    Nanocatalase-Based Oxygen-Generating Nanocarriers for Active Oxygen Delivery to Relieve Hypoxia in Pancreatic Cancer

    DOI: 10.1021/acsanm.2c04241

    Sample: PANC-1 cells
  10. ARCHIVES OF TOXICOLOGY (2023) IF: 6.168
    The circadian rhythm gene Bmal1 ameliorates acute deoxynivalenol-induced liver damage

    DOI: 10.1007/s00204-022-03431-x

    Sample: liver,serum
  11. Antioxidants (2022) IF: 6.313
    Evaluating the Nutritional and Immune Potentiating Characteristics of Unfermented and Fermented Turmeric Camel Milk in Cyclophosphamide-Induced Immunosuppression in Rats

    DOI: 10.3390/antiox11040792

    PMID: 35453477

    Sample: blood
  12. Antioxidants (2022) IF: 6.313
    Antioxidative, Antidiabetic, and Hypolipidemic Properties of Probiotic-Enriched Fermented Camel Milk Combined with Salvia officinalis Leaves Hydroalcoholic Extract in Streptozotocin-Induced Diabetes in Rats

    DOI: 10.3390/antiox11040668

    PMID: 35453353

    Sample: serum
  13. Antioxidants (2022) IF: 6.313
    Ameliorative and Antioxidative Potential of Lactobacillus plantarum-Fermented Oat (Avena sativa) and Fermented Oat Supplemented with Sidr Honey against Streptozotocin-Induced Type 2 Diabetes in Rats

    DOI: 10.3390/antiox11061122

    Sample: serum
  14. LIFE SCIENCES (2021) IF: 5.037
    Protective effects of low-temperature plasma on cisplatin-induced nephrotoxicity

    DOI: 10.1016/j.lfs.2021.120230

    PMID: 34919900

    Sample: kidney tissue
  15. Fermentation-Basel (2022) IF: 5.123
    Antidiabetic and Hypolipidemic Efficiency of Lactobacillus plantarum Fermented Oat (Avena sativa) Extract in Streptozotocin-Induced Diabetes in Rats

    DOI: 10.3390/fermentation8060267

    Sample: serum
  16. CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
    In vitro and in vivo anticancer effects of syringic acid on colorectal cancer: Possible mechanistic view

    DOI: 10.1016/j.cbi.2020.109337

    PMID: 33548266

    Sample: Cell sample
  17. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079

    Sample: livers
  18. LIVER TRANSPLANTATION (2022) IF: 5.799
    Cerium oxide nanoparticles administration during machine perfusion of discarded human livers: a pilot study

    DOI: 10.1002/lt.26421

    PMID: 35100468

    Sample: liver
  19. Frontiers in Pharmacology (2022) IF: 5.988
    Anti-arthritic and toxicological evaluation of ethanolic extract of Alternanthera bettzickiana in rats.

    DOI: 10.3389/fphar.2022.1002037

    Sample: serum
  20. BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 4.081
    Selenium Supplementation Protects Against Arsenic-Trioxide-Induced Cardiotoxicity Via Reducing Oxidative Stress and Inflammation Through Increasing NAD+ Pool

    DOI: 10.1007/s12011-022-03478-y

    Sample: heart tissue
  21. NEUROCHEMISTRY INTERNATIONAL (2022) IF: 4.297
    Calcium channel antagonists interfere with the mechanism of action of elastin-derived peptide VGVAPG in mouse cortical astrocytes in vitro

    DOI: 10.1016/j.neuint.2022.105405

    PMID: 35934159

    Sample: astrocyte cell
  22. Drug Design Development and Therapy (2022) IF: 4.319
    The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

    DOI: 10.2147/DDDT.S344247

    PMID: 35965961

    Sample: serum
  23. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    Sample: serum
  24. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2019) IF: 4.527
    Chronic neurodegeneration by aflatoxin B1 depends on alterations of brain enzyme activity and immunoexpression of astrocyte in male rats

    DOI: 10.1016/j.ecoenv.2019.109407

    PMID: 31279280

    Sample: Tissue homogenate
  25. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats

    DOI: 10.1016/j.biopha.2020.109841

    PMID: 31972360

    Sample: Tissue homogenate
  26. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats

    DOI: 10.1016/j.procbio.2022.10.004

    Sample: liver,kidney,lungs
  27. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Optimization of ultrasound-assisted extraction, structural, functional, and antioxidant properties of Glycyrrhiza uralensis seed protein

    DOI: 10.1016/j.procbio.2022.11.001

    Sample: serum,liver
  28. MOLECULES (2022) IF: 4.927
    Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

    DOI: 10.3390/molecules27196470

    PMID: 36235007

    Sample: plasma
  29. KIDNEY & BLOOD PRESSURE RESEARCH (2023) IF: 3.096
    Protective effects of Xanthohumol against diabetic nephropathy in a mouse model

    DOI: 10.1159/000528650

    Sample: kidney
  30. EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
    Intraocular pressure-lowering effects of imidazo[1,2-a]- and pyrimido[1,2-a]benzimidazole compounds in rats with dexamethasone-induced ocular hypertension

    DOI: 10.1016/j.ejphar.2019.01.059

    Sample: Tissue homogenate
  31. Animals (2022) IF: 3.231
    Milk Pathogens in Correlation with Inflammatory, Oxidative and Nitrosative Stress Markers in Goat Subclinical Mastitis

    DOI: 10.3390/ani12233245

    Sample: skimmed milk
  32. NEUROTOXICITY RESEARCH (2019) IF: 3.311
    The Elastin-Derived Peptide VGVAPG Does Not Activate the Inflammatory Process in Mouse Cortical Astrocytes In Vitro

    DOI: 10.1007/s12640-019-00114-x

    Sample: mouse astrocyte cell
  33. NEUROTOXICITY RESEARCH (2019) IF: 3.311
    Antiproliferative Effect of Elastin-Derived Peptide VGVAPG on SH-SY5Y Neuroblastoma Cells

    DOI: 10.1007/s12640-019-00040-y

    PMID: 31161598

    Sample: SH-SY5Y cells
  34. ANIMAL FEED SCIENCE AND TECHNOLOGY (2022) IF: 3.313
    Housefly maggot meal complement soybean meal in a fish-free diet for hybrid catfish (Clarias gariepinus ♀ x Heterobranchus longifilis ♂): Effect on growth, body composition, blood biochemistry and antioxidant enzyme activity

    DOI: 10.1016/j.anifeedsci.2022.115543

    Sample: serum
  35. Processes (2022) IF: 3.352
    Gastroprotective, Biochemical, and Acute Toxicity Effects of Papaver decaisnei against Ethanol-Induced Gastric Ulcers in Rats

    DOI: 10.3390/pr10101985

    Sample: gastric tissue
  36. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY (2019) IF: 3.377
    -arginine reverses aging process through suppression of oxidative stress, inflammation, and apoptosis in the rat heart

    DOI: 10.1007/s00424-019-02311-1

    Sample: Tissue homogenate
  37. International Journal of Environmental Research and Public Health (2021) IF: 3.39
    Polystyrene Microplastics Exposure: An Insight into Multiple Organ Histological Alterations, Oxidative Stress and Neurotoxicity in Javanese Medaka Fish (Oryzias javanicus Bleeker, 1854)

    DOI: 10.3390/ijerph18189449

    Sample: intestine,brain
  38. METABOLIC BRAIN DISEASE (2022) IF: 3.584
    Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats

    DOI: 10.1007/s11011-022-00952-3

    PMID: 35298730

    Sample: hippocampal,hepatic
  39. SAUDI PHARMACEUTICAL JOURNAL (2019) IF: 3.643
    Propolis ameliorates cerebral injury in focal cerebral ischemia/reperfusion (I/R) rat model via upregulation of TGF-β1

    DOI: 10.1016/j.jsps.2019.11.013

    PMID: 31920438

    Sample: Tissue homogenate
  40. LIFE SCIENCES (2020) IF: 3.647
    Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

    DOI: 10.1016/j.lfs.2020.118876

    PMID: 33310035

    Sample: cell
  41. PLANT PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 3.72
    Overexpression of ethylene response factor ERF96 gene enhances selenium tolerance in Arabidopsis

    DOI: 10.1016/j.plaphy.2020.02.024

    PMID: 32097848

    Sample: Plant Tissue
  42. JOURNAL OF PHARMACY AND PHARMACOLOGY (2020) IF: 2.571
    By reducing oxidative stress, naringenin mitigates hyperglycaemia‐induced upregulation of hepatic nuclear factor erythroid 2‐related factor 2 protein

    DOI: 10.1111/jphp.13319

    Sample: Cell lysate
  43. MICROBIAL PATHOGENESIS (2019) IF: 2.581
    Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

    DOI: 10.1016/j.micpath.2019.103557

    Sample: small intestinal tissues
  44. BIOLOGICAL TRACE ELEMENT RESEARCH (2020) IF: 2.639
    Impact of Coenzyme Q10 and Selenium on Seminal Fluid Parameters and Antioxidant Status in Men with Idiopathic Infertility

    DOI: 10.1007/s12011-020-02251-3

    Sample: Semen
  45. Applied Sciences-Basel (2021) IF: 2.679
    Biochemical Mapping of the Inflamed Human Dental Pulp

    DOI: 10.3390/app112110395

    Sample: dental pulp lysates
  46. Toxicology Research (2022) IF: 2.68
    Glutathione attenuated lambda-cyhalothrin-induced alteration of serum total cholesterol concentration and oxidative stress parameters in rats

    DOI: 10.1093/toxres/tfac080

    Sample: serum
  47. ECOTOXICOLOGY (2021) IF: 2.823
    Garlic (Allium sativum) and Fu-ling (Poria cocos) mitigate lead toxicity by improving antioxidant defense mechanisms and chelating ability in the liver of grass carp (Ctenopharyngodon idella)

    DOI: 10.1007/s10646-021-02405-6

    PMID: 33830385

    Sample: Tissue homogenate
  48. BMC Complementary and Alternative Medicine (2020) IF: 2.833
    HPLC-DAD analysis of Hyssopus Cuspidatus Boriss extract and mensuration of its antioxygenation property

    DOI: 10.1186/s12906-020-03016-0

    PMID: 32689984

    Sample: Plant Fluid
  49. CURRENT ISSUES IN MOLECULAR BIOLOGY (2023) IF: 2.976
    Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

    DOI: 10.3390/cimb45020057

    Sample: colonic tissue
  50. Brazilian Journal of Pharmaceutical Sciences (2022) IF: 1.214
    Stingless bee propolis, metformin, and their combination alleviate diabetic cardiomyopathy

    DOI: 10.1590/s2175-97902022e19652

    Sample: Heart tissue
  51. Experimental and Therapeutic Medicine (2018) IF: 1.41
    Rutin inhibits coronary heart disease through ERK1/2 and Akt signaling in a porcine model

    DOI: 10.3892/etm.2017.5365

    PMID: 29250160

    Sample: Urine
  52. HORTSCIENCE (2021) IF: 1.455
    Effects of Salt Stress on Photosynthetic Fluorescence Characteristics, Antioxidant System, and Osmoregulation of Coreopsis tinctoria Nutt.

    DOI: 10.21273/HORTSCI15956-21

    Sample: seed
  53. JOURNAL OF FOOD BIOCHEMISTRY (2020) IF: 1.662
    HPLC analysis, cytotoxicity, and safety study of Moringa oleifera Lam. (wild type) leaf extract

    DOI: 10.1111/jfbc.13400

    PMID: 32729119

    Sample: Plasma
  54. TOXICOLOGY AND INDUSTRIAL HEALTH (2020) IF: 1.708
    Elevated, sustained, and yet reversible biotoxicity effects of lead on cessation of exposure: Melatonin is a potent therapeutic option:

    DOI: 10.1177/0748233720937199

    Sample: Plasma
  55. ANDROLOGIA (2020) IF: 1.951
    Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro

    DOI: 10.1111/and.13825

    PMID: 32926461

    Sample: Cell lysate
  56. International Journal of Inflammation (2020)
    Therapeutic Effects of the Combination of Alpha-Lipoic Acid (ALA) and Coenzyme Q10 (CoQ10) on Cisplatin-Induced Nephrotoxicity

    DOI: 10.1155/2020/5369797

    PMID: 32328233

    Sample: Tissue homogenate
  57. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009

    Sample: plasma
  58. Journal of Nutrition and Metabolism (2020)
    Digestive Fermentation, Antioxidant Status, and Haemato-Biochemical Indices of Growing Rabbits Fed on Diets Supplemented with Ziziphus spina-christi Leaf

    DOI: 10.1155/2020/9046862

    PMID: 32318290

    Sample: Serum
  59. Toxicology Reports (2017)
    Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

    DOI: 10.1016/j.toxrep.2017.12.002

    Sample: plasma
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

M*****aSubmitted [ Aug 15 2022 ]

Asked: Would you give us any additional advice on the cell pretreatment method ?

Reply

A

adminSubmitted [ Aug 15 2022 ]

Answered: Collect the cells and wash the cells with PBS (0.01 M, pH 7.4) for 1~2 times. Centrifuge at 1000 g for 10 min and then discard the supernatant and keep the cell sediment. Add homogenization medium at a ratio of cell number (10^6): homogenization medium (μL) =1: 300. Sonicate or grind with hand-operated in ice water bath. Centrifuge at 1500 g for 10 min, then take the supernatant and preserve it on ice for detection. Meanwhile, determine the protein concentration of supernatant (E-BC-K318-M). If not detected on the same day, the cells sample (without homogenization) can be stored at -80℃ for a month.

Q

M*****aSubmitted [ Aug 15 2022 ]

Asked: E-BC-K031-S Can the kits be used on plant tissues? If yes, what is the volume sample details?

Reply

A

adminSubmitted [ Aug 15 2022 ]

Answered: Yes, it can be used on plant tissues, the volume of tissue homogenate is 0.05 mL

Q

K******aSubmitted [ Jul 01 2022 ]

Asked: Can the kits be used on plant tissues? If yes, what is the volume sample details?

Reply

A

adminSubmitted [ Jul 01 2022 ]

Answered: Yes , the kit can be uesd on plant tissues. Sample tube: Add a* mL of sample and 1 mL of Reagent 1 into the 5 mL EP tubes. Note:For the cell homogenate and tissue homogenate, a* is 0.05 mL.

Q

C*******aSubmitted [ Apr 12 2022 ]

Asked: Can I use this kit on cell culture supernatant and homogenised cell pellet?

Reply

A

adminSubmitted [ Apr 12 2022 ]

Answered: E-BC-K031-S Catalase (CAT) Activity Assay Kit can detect homogenized cell pellet but cannot detect cell culture supernatant.

Q

S***********eSubmitted [ Dec 01 2021 ]

Asked: Hi. Thank you for your answer. But I couldn\'t read the mail by my mistake and deleted it. I\'m really sorry, but can you send me an answer again? Not only catalase kits but also E-BC-K136-S, E-BC-K284-S, Are E-BC-K227-S, E-BC-K353-S, E-BC-K099-S, and E-BC-K138-S kit also capable of plant analysis? If possible, I would appreciate it if you could let me know the preprocessing. I\'m sorry I have so many questions and I\'m waiting for your answer. thank you have a good day!

Reply

A

adminSubmitted [ Dec 01 2021 ]

Answered: Thanks for your prompt reply. Could you let me know your email? It will be more convenient for me to send you related document.

Q

S***********eSubmitted [ Nov 30 2021 ]

Asked: We are Korea Institute of Toxicology in south korea I want to use Elabscience\'s kit. Can I do plant analysis with the Catalase Assay Kit? If yes, I\'m curious about the pre-processing method.

Reply

A

adminSubmitted [ Nov 30 2021 ]

Answered: It is OK to do plant analysis with the Catalase Assay Kit. There is details information in the manual of this item, including the pre-processing method. You are able to download it from our website directly. https://file.elabscience.com/Manual/biochemical_kits/E-BC-K031-S.pdf

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