Current Position:Home>>Kits>>Metabolism Assays>> Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

Cat.No.:E-BC-K125-M
Detection instrument: Microplate reader(324 nm)

Detection method: Colorimetric method

To Purchase E-BC-K125-M

Size:

96T
48T

Price: $280

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.

Performance characteristics

Synonyms	ChAT
Sample type	Animal tissue
Sensitivity	1.21 U/g fresh weigh
Detection range	1.21-40 U/g fresh we
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	65 min
Precision	Average inter-assay CV: 9.400%Average intra-assay CV: 5.100%
Other instruments required	Test tube, Centrifuge, 100℃ Water bath, Incubator.
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 2, Reagent 3, Reagent 6: -20℃; Others: 2-8℃
Valid period	12 months

Images

The activity of ChAT was reduced by the scopolamine injection, and recovered in Scop+AmpA group.

Y Hong et al investigate the mechanism of ampelopsin A in ameliorating cognitive and memory function. Choline Acetyltransferase (ChAT) activity of mouse hippocampus was determined using ChAT activity assay kit (E-BC-K125-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.21-40 U/g fresh weight).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
20% Mouse brain tissue homogenate	1-2
20% Rat heart tissue homogenate	1
20% Rat liver tissue homogenate	1
20% Mouse kidney tissue homogenate	1
20% Rat lung tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Processes (2023) IF: 3.352
Memory-Improving Activity of the Flower Extract from Chrysanthemum boreale (Makino) Maskino in Scopolamine-Treated Rodents

DOI: 10.3390/pr11010159

Sample: Hippocampal

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

Cat. No	E-BC-K125-M
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha