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Cholinesterase (CHE) Activity Assay Kit

Cat.No.:E-BC-K052-S
Detection instrument: Spectrophotometer(520 nm)

Detection method: Colorimetric method

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Detection principle

Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with basic hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated.

Performance characteristics

Synonyms	CHE
Sample type	Whole blood,serum,plasma,tissue,cells
Sensitivity	1.17 U/mL
Detection range	1.17-160 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 9.400%Average intra-assay CV: 3.700%
Other instruments required	Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.17-160 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	2-3
Human plasma	2-3
Mouse serum	2-3
Mouse plasma	2-3
SH-SY5Ycells	1
10% Mouse brain tissue homogenate	1
10% Rat brain tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Mouse brain tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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