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Citric Acid (CA) Colorimetric Assay Kit

Cat:E-BC-K351-S
Manual MSDS

Price: $ 220

Price: $ 150

Size:
100Assays 50Assays
Quantity:
  • Sample Type: tissue;mitochondria;other liquid samples
  • Detection Instrument: Spectrophotometer(545 nm)
  • Reasearch Areas: Mitochondria Function;Tricarboxylic Acid Cycle
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Product Details
Detection Principle In acidic condition, Cr (Ⅵ) will be reduced to Cr3+, Cr3+ reacts with citric acid. And the product has a characteristic absorption peak at 545 nm, therefore the content of citric acid in sample can be calculated by measuring the absorbance value at 545 nm.
Synonyms CA
Sample Type tissue,mitochondria,other liquid samples
Sensitivity 0.05 mmol/L
Detection Range 0.05-5.0 mmol/L
Detection Method Colorimetric method
Assay Time 60 min
Precision inter-assay CV: 5.4% | intra-assay CV: 4.2%
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 4-6
10% Mouse kidney tissue homogenate 2-4
10% Rat kidney tissue homogenate 1
10% Mouse heart tissue homogenate 2-4
10% Mouse brain tissue homogenate 1
10% Mouse liver tissue homogenate 1
The diluent is buffer solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.