Citric Acid (CA) Colorimetric Assay Kit (E-BC-K351-M)
                        For research use only.
| Detection Principle | In acidic condition, Cr (Ⅵ) will be reduced to Cr3+, Cr3+ reacts with citric acid. And the product has a characteristic absorption peak at 545 nm, therefore the content of citric acid in sample can be calculated by measuring the absorbance value at 545 nm. | 
| Synonyms | CA | 
| Sample Type | Serum,Plasma,Animal tissue,Mitochondria | 
| Detection Method | Colorimetric method | 
| Detection Instrument | Microplate reader (535-555 nm, optimum wavelength: 545 nm) | 
| Research Area | Mitochondria Function , Tricarboxylic Acid(TCA)Cycle , Energy Metabolism | 
| Storage | This product can be stored at 2-8°C for 12 months with shading light. | 
| Valid Period | 12 months | 
| Sensitivity | 0.06 mmol/L | 
| Detection Range | 0.06-2.0 mmol/L | 
| Precision | inter-assay CV: 4% | intra-assay CV: 4% | 
| Sample Volume | 0.1 mL | 
| Assay Time | 50 min | 
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor | 
|---|---|
| Human serum | 5-20 | 
| Dog serum | 5-10 | 
| Rat serum | 10-20 | 
| Horse serum | 5-10 | 
| Mouse plasma | 5-20 | 
| 10% Rat brain tissue homogenate | 5-10 | 
| 10% Rat liver tissue homogenate | 5-20 | 
| 10% Rat kidney tissue homogenate | 5-10 | 
| 10% Rat lung tissue homogenate | 15-30 | 
| 10% Mouse heart tissue homogenate | 5-20 | 
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
{{antibodyDetailsPage.numTotal}} Results
- 
                                    
                                    
                                    {{item.title}}
                                    
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.:{{item.cat}}
{{index}} {{goods_show_value}}
 
Other Formats
{{formatDetailsPage.numTotal}} Results
- 
                                    
                                    
                                    {{item.title}}
                                    
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.:{{item.cat}}
{{index}} {{goods_show_value}}
 
- 
                                    
                                    
IF:{{item.impact}}
Journal:{{item.journal}} ({{item.year}})
DOI:{{item.doi}}Reactivity:{{item.species}}
Sample Type:{{item.organization}}
 
- 
                                    
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
 
                            
