CST3 Monoclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Mouse
- Reactivity: Human
- Applications: IHC-p
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Verified Samples |
Verified Samples in IHC:Human brain |
Dilution |
IHC 1:100-200 Western Blot Operation Guide |
Clonality | Monoclonal |
Immunogen | Recombinant Protein of Cystatin C of CST3 |
Abbre | CST3 |
Synonyms | AD 8;AD8;Amyloid angiopathy and cerebral hemorrhage;ARMD11;bA218C14.4 (cystatin C);bA218C14.4;Cst 3;Cst3;CST3 protein;Cystatin 3;Cystatin-3;Cystatin-C;Cystatin3;CystatinC;CYTC;Epididymis secretory protein Li 2;Gamma trace;Gamma-trace;HCCAA;HEL S 2;MGC117328;Neuroendocrine basic polypeptide;Post gamma globulin;Post-gamma-globulin |
Swissprot | |
Observed MW |
14kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Secreted. |
Tissue Specificity | Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. |
Concentration | 1 mg/mL |
Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
Purification Method | Protein A purification |
Research Areas | Cancer; Cell Biology; Cardiovascular; Stem Cells; Tags and Cell Markers |
Clone No. | Clone:5A2 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Biological ice pack at 4 ℃ |
background | As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L. |