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CST3 Monoclonal Antibody

Cat:E-AB-22217
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Mouse
  • Reactivity: Human
  • Applications: IHC-p
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Product Details
Verified Samples Verified Samples in IHC:Human brain
Dilution

IHC 1:100-200

Western Blot Operation Guide
Clonality Monoclonal
Immunogen Recombinant Protein of Cystatin C of CST3
Abbre CST3
Synonyms AD 8;AD8;Amyloid angiopathy and cerebral hemorrhage;ARMD11;bA218C14.4 (cystatin C);bA218C14.4;Cst 3;Cst3;CST3 protein;Cystatin 3;Cystatin-3;Cystatin-C;Cystatin3;CystatinC;CYTC;Epididymis secretory protein Li 2;Gamma trace;Gamma-trace;HCCAA;HEL S 2;MGC117328;Neuroendocrine basic polypeptide;Post gamma globulin;Post-gamma-globulin
Swissprot
Observed MW 14kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted.
Tissue Specificity Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method Protein A purification
Research Areas Cancer; Cell Biology; Cardiovascular; Stem Cells; Tags and Cell Markers
Clone No. Clone:5A2
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Biological ice pack at 4 ℃
background As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.