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Cysteine (Cys) Colorimetric Assay Kit

Cat.No.:E-BC-K352-M
Detection instrument: Microplate reader(600-620 nm,optimum wavelength: 600 nm)

Detection method: Colorimetric method

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96T
48T

Price: $380

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Protocols for metabolism assays

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Detection principle

Phosphotungstic acid can be reduced by Cys and form tungsten blue, which has an absorption peak at 600 nm. Cys content can be calculated with the absorbance at 600 nm.

Performance characteristics

Synonyms	Cys
Sample type	Serum,plasma,animal tissue,cells
Sensitivity	0.03 mmol/L
Detection range	0.07-2.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 1.300%Average intra-assay CV: 1.100%
Other instruments required	Micropipettor, Centrifuge, Water bath, Incubator, Vortex mixer
Other reagents required	Phosphoric acid
Storage	2-8℃
Valid period	12 months

Images

The concentration of cysteine was reduced by adding boric acid with dose dependent.

F K Coban et al investigate the acetaminophen-induced nephrotoxicity suppressed by boric acid. Cysteine (CYS) levels in rat serum were determined using CYS colorimetric assay kit (E-BC-K352-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.07-2.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
10% Rat lung tissue homogenate	1
10% Mouse heart tissue homogenate	1
10% Rat brain tissue homogenate	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Science Advances (2022) IF: 14.957
d-Serine controls epidermal vesicle release via NMDA receptor, allowing tissue migration during the metamorphosis of the chordate Ciona

DOI: 10.1126/sciadv.abn3264
Cancer Cell International (2023) IF: 6.429
Coordinated reprogramming of renal cancer transcriptome, metabolome and secretome associates with immune tumor infiltration

DOI: 10.1186/s12935-022-02845-y

Sample: KIJ265T cell,KIJ308T cell,RPTEC/TERT1 cell,Caki-1
HUMAN & EXPERIMENTAL TOXICOLOGY (2022) IF: 3.247
The effects of acrylamide-mediated dorsal root ganglion neurons injury on ferroptosis.

DOI: 10.1177/09603271221129786

Sample: dorsal root ganglia (DRG) neurons

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