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D-Xylose Colorimetric Assay Kit

Cat.No.:E-BC-K018-S
Detection instrument: Spectrophotometer(554 nm)

Detection method: Colorimetric method

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Detection principle

D-xylose can produce furfural by dehydration in strong acid solution. The generated furfural reacts with Phloroglucinol to form pink compounds. The content of D-xylose can be calculated by colorimetric assay at 554 nm.

Performance characteristics

Synonyms	D-aldopentose
Sample type	Serum,plasma,urine
Sensitivity	0.007 mmol/L
Detection range	0.007-4 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	30 min
Precision	Average inter-assay CV: 4.500%Average intra-assay CV: 2.200%
Other instruments required	Micropipettor, Vortex mixer, Water bath
Other reagents required	Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

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Collection and treatment of common samples for metabolism assay detection

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