DEX (Dexamethasone) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E009

      Size:
      • 96T
      Qty:
      - +
      Price: $582

      Sensitivity: 0.10 ppb

      Sample type: Tissue, Milk, Feed

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Indirect-Competitive-ELISA as the method. It can detect Dexamethasone (DEX) in samples, such as Muscle tissue, milk etc. This kit is composed of ELISA Microtiter plate , HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with DEX. During the reaction, DEX in the samples or standard competes with DEX on the solid phase supporter for sites of DEX antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of DEX. The concentration of DEX in the samples can be calculated by comparing the OD of the samples to the standard curve.

       

      Technical indicator

      Sensitivity: 0.1 ppb (ng/ml)

      Reaction mode: 25℃,30 min30 min15 min

      Detection limit: Muscle tissue ---0.2 ppb, milk---0.5 ppb, fedd---1 ppb

      Cross-reactivity: DEX ---100%

      Sample recovery rate: Tissue/ milk / fodder ---80%±15%, 

       

      Kits components

       

      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1 mL each

      (0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb)

      High Concentrated Standard (100 ppb)

      1 mL

      HRP Conjugate 

      11 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A 

      6 mL

      Substrate Reagent B 

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      Reconstitution Buffer

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other supplies required

      Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators/Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: Acetic ether, NaOH, N-hexane.


      Assay procedure
      Centrifuge the sample again after thawing before the assay. Bring all reagents to room temperature before use. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming.
      1. Number: number the samples and standard in order (multiple well), and keep a record of standard wells and sample wells.
      2. Add sample: add 100 μL of Standard or Samples per well, then add 50 μL of antibody working solution, cover the plate with sealer, oscillate for 5 s gently to mix thoroughly, incubate for 30 min at 25℃ in the dark.
      3. Wash: uncover the sealer carefully, remove the liquid in each well. Immediately add 300 μL of wash buffer to each well and wash. Repeat wash procedure for 5 times, 30 s intervals/time. Invert the plate and pat it against thick clean absorbent paper (If bubbles exist in the wells, clean tips can be used to prick them).
      4. Add HRP conjugate: Add 100 μL of HRP Conjugate to each well, incubate for 30 min at 25℃ in the dark.
      5. Wash: Repeat step 3
      6. Color Development: add 50 μL of substrate solution A to each well, and then add 50 μL of substrate solution B. Gently oscillate for 5 s to mix thoroughly. Incubate with shading light for 15 min at 25℃ (The reaction time can be extended according to the actual color change).
      7. Stop reaction: add 50 μL of stop solution to each well, oscillate gently to mix thoroughly.
      8. OD Measurement: determine the absorbance (OD value) of each well at 450 nm with a micro-plate reader (the 450/630 nm double wavelength is recommended). This step should be finished in 10 min after stop reaction.




      Storage and valid period

      Storage: Store at 2-8. Avoid freeze / thaw cycles.

      Valid Period: 1 year, expiration  date is on the packing box.


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