Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Elab Fluor® 488 Anti-Human CD45RA Antibody[HI100]
Cat.No.:E-AB-F1052L
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Alternate Names | Receptor-type tyrosine-protein phosphatase C,Ptprc,L-CA,Ly-5,T200,CD45 |
Swissprot | P08575 |
References |
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85. |
Form | Liquid |
Clone No. | HI100(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG2b, κ |
Isotype Control | Elab Fluor® 488 Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812L] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Elab Fluor® 488 |
Elab Fluor® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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