Alternate Names	Receptor-type tyrosine-protein phosphatase C,Ptprc,L-CA,Ly-5,T200,CD45
Swissprot	P08575
References	1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Product Details

Form	Liquid
Clone No.	HI100(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2b, κ
Isotype Control	Elab Fluor^® 488 Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812L]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 488
	Elab Fluor^® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Biotin Anti-Human CD45RA Antibody[HI100]	25μg, 100μg	Check More
FITC Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD45RA Antibody[HI100]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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