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Elab Fluor® 488 Anti-Human CD57 Antibody[HNK-1]

  • Cat.No.:E-AB-F1067L

  • Applications: FCM
  • Isotype: Mouse IgM, κ
  • Host: Mouse

To Purchase E-AB-F1067L

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $100
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Antigen Details

Alternate Names HNK-1, NK-1, Leu-7
References
1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711-714.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M, et al. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.

Product Details

Form Liquid
Clone No. HNK-1(See Other Available Formats)
Host Mouse
Isotype Mouse IgM, κ
Isotype Control Elab Fluor® 488 Mouse IgM, κ Isotype Control[MM-30] [Product E-AB-F09782L]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Elab Fluor® 488
Elab Fluor® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD57, also known as HNK-1, NK-1, and Leu-7 is a 100-115 kD oligosaccharide antigenic determinant expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. CD57 is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, and is also expressed on neural cells and striated muscle. CD57 is not expressed on red blood cells, granulocytes, monocytes, or platelets. While the function of CD57 is unknown, binding to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances (AIDS, autoimmune disease, viral infections, and allograft transplants).

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
Purified Anti-Human CD57 Antibody[HNK-1] 25μg, 100μg Check More
Biotin Anti-Human CD57 Antibody[HNK-1] 25μg, 100μg Check More
FITC Anti-Human CD57 Antibody[HNK-1] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD57 Antibody[HNK-1] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD57 Antibody[HNK-1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human CD57 Antibody[HNK-1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Human CD57 Antibody[HNK-1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Human CD57 Antibody[HNK-1] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human CD57 Antibody[HNK-1] 20Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Human CD57 Antibody[HNK-1] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Human CD57 Antibody[HNK-1] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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