Alternate Names	L-selectin,Sell,CD62 antigen-like family member L,LAM-1,LECAM1,Lymph node homing receptor,Ly-22, CD62L,Lnhr,Ly22
Swissprot	P14151
References	1. Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. 2. Kishimoto T, et al. 1991. Blood 78:805.

Product Details

Form	Liquid
Clone No.	DREG56(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	Elab Fluor^® 488 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792L]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® 488
	Elab Fluor^® 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520 nm (e.g., a 525/40 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human CD62L Antibody[DREG56]	25μg, 100μg	Check More
Biotin Anti-Human CD62L Antibody[DREG56]	25μg, 100μg	Check More
FITC Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Human CD62L Antibody[DREG56]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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