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|Alternate Names||HNK-1, NK-1, Leu-7|
1. Schubert J, et al. 1989. In Leucocyte Typing IV (Knapp W, ed) Oxford University Press Oxford pp 711-714.
2. Palmer BE, et al. 2005. J. Immunol. 175:8415.
3. Schachner M, et al. 1995. Trends Neurosci. 18:183.
4. Wood KL, et al. 2005. Clin. Immunol. 117:294.
|Clone No.||HNK-1(See Other Available Formats)|
|Isotype||Mouse IgM, κ|
|Isotype Control||Elab Fluor® Violet 450 Mouse IgM, κ Isotype Control[MM-30] [Product E-AB-F09782Q]|
|Application||FCM (See the Application Description)|
|Storage Buffer||Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.|
|Recommended Use||Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.|
|Shipping||Biological ice pack at 4℃|
|Stability & Storage||Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
|Conjugation||Elab Fluor® Violet 450|
|Elab Fluor® Violet 450 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450 nm (e.g., a 450/45 nm bandpass filter).|
How to select the appropriate detection channel through the spectrogram?
|CD57, also known as HNK-1, NK-1, and Leu-7 is a 100-115 kD oligosaccharide antigenic determinant expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans. CD57 is expressed on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, and is also expressed on neural cells and striated muscle. CD57 is not expressed on red blood cells, granulocytes, monocytes, or platelets. While the function of CD57 is unknown, binding to L-selectin, P-selectin, and a fragment of laminin suggests that CD57 may be involved in cell-matrix interactions. CD57 is increased in some disease states associated with CD4/CD8 imbalances (AIDS, autoimmune disease, viral infections, and allograft transplants).|
|Related Protocols||Staining Cell Surface Targets for Flow Cytometry|
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