Alternate Names	T-cell surface glycoprotein CD3 δ, γ, ε, and ζ chains,CD3 Complex, T3,CD3
Swissprot	P19377,Q64159,D4A5M2
Gene ID	25710,300678,315609,25300
References	1. Tanaka T, et al. 1989 J. Immunol. 142:2791. 2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.

Product Details

Form	Liquid
Clone No.	G4.18(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG3, κ
Isotype Control	Elab Fluor^® Red 780 Mouse IgG3, κ Isotype Control[A112-3] [Product E-AB-F09752S]
Reactivity	Rat
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Elab Fluor^® Red 780
	Elab Fluor^® Red 780 is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 770 nm (e.g., a 780/60 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Rat CD3 Antibody[G4.18]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Rat CD3 Antibody[G4.18]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Rat CD3 Antibody[G4.18]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Rat CD3 Antibody[G4.18]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Rat CD3 Antibody[G4.18]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Rat CD3 Antibody[G4.18]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Rat CD3 Antibody[G4.18]	25μg, 100μg	Check More
PE Anti-Rat CD3 Antibody[G4.18]	25μg, 100μg	Check More
APC Anti-Rat CD3 Antibody[G4.18]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Rat CD3 Antibody[G4.18]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Rat CD3 Antibody[G4.18]	25μg, 100μg	Check More

Citations

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