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Ethanol Colorimetric Assay Kit

Cat:E-BC-K891-M
Manual MSDS

Price: $ 380

Price: $ 260

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;wine
  • Detection Instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)
  • Reasearch Areas: Liver Renal function biomarkers
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For research use only. Order now, ship in 3 days

Product Details
Detection Principle Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm.
Sample Type serum,plasma,wine
Sensitivity 0.27 μmol/mL
Detection Range 0.27–10.0 μmol/mL
Detection Method Colorimetric method
Assay Time 30 min
Precision inter-assay CV: 5% | intra-assay CV: 3.5%
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Beer (2.8% alcohol) 60-100
White wine (12% alcohol) 250-300
The diluent is double distilled water. For the dilution of other sample types, please do pretest to confirm the dilution factor.