Ethanol Colorimetric Assay Kit
Price: $ 380
Price: $ 260
Size:
96T
48T
Quantity:
- Sample Type: serum;plasma;wine
- Detection Instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)
- Reasearch Areas: Liver Renal function biomarkers
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For research use only. Order now, ship in 3 days
Product Details
Detection Principle | Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm. | ||||||
Sample Type | serum,plasma,wine | ||||||
Sensitivity | 0.27 μmol/mL | ||||||
Detection Range | 0.27–10.0 μmol/mL | ||||||
Detection Method | Colorimetric method | ||||||
Assay Time | 30 min | ||||||
Precision | inter-assay CV: 5% | intra-assay CV: 3.5% | ||||||
Storage | This product can be stored at -20°C for 12 months with shading light. | ||||||
Valid Period | 12 months | ||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is double distilled water. For the dilution of other sample types, please do pretest to confirm the dilution factor. |