Ethanol Colorimetric Assay Kit (E-BC-K891-M)
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For research use only.
| Detection Principle | Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm. |
| Sample Type | Serum,Plasma,Wine |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.27 μmol/mL |
| Detection Range | 0.27-10.0 μmol/mL |
| Precision | inter-assay CV: 5% | intra-assay CV: 3.5% |
| Sample Volume | 40 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Beer (2.8% alcohol) | 60-100 |
| White wine (12% alcohol) | 250-300 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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