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FITC Anti-Mouse CD86 Antibody[GL-1]

Uniprot : P42082
  • Cat.No.:E-AB-F0994UC

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F0994UC

Size:
  • 25μg
  • 100μg
Price: $54
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Antigen Details

Alternate Names T-lymphocyte activation antigen CD86,Cd86,Activation B7-2 antigen,Early T-cell costimulatory molecule 1,ETC-1
Swissprot P42082
References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Product Details

Form Liquid
Clone No. GL-1
Host Rat
Isotype Rat IgG2a, κ
Isotype Control FITC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833C]
Reactivity Mouse
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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