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FITC Anti-Mouse Perforin Antibody[S16009A]

Uniprot : P10820
  • Cat.No.:E-AB-F1294UC

  • Applications: ICFCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1294UC

Size:
  • 25μg
  • 100μg
Price: $100
Qty:

Antigen Details

Alternate Names Prf1, Pfp, Pfn, Pfn-1,Cytolysin,Lymphocyte pore-forming protein
Swissprot P10820
Gene ID 18646 
References
1.Podack ER, et al. 1988. Immunol. Rev. 103:203.
2.Konjar S, et al. 2010. Immunology. 131:257.
3.Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735.
4.Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.

Product Details

Form Liquid
Clone No. S16009A(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control FITC Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833C]
Reactivity Mouse
Application ICFCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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