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Free Fatty Acids (FFA) Fluorometric Assay Kit

  • Cat.No.:E-BC-F039

  • Detection instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)

To Purchase E-BC-F039

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  • 96T
  • 48T
Price: $450
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Detection principle

Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

Performance characteristics

Synonyms FFA/NEFA
Sample type Serum, plasma, animal tissue
Sensitivity 0.58 μmol/L
Detection range 0.58-20 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 70 min
Precision Average inter-assay CV: 5.3%Average intra-assay CV: 4.2%
Other instruments required Micropipette, Vortex mixer, Centrifuge
Storage -20℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-20 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

10-20

Rat serum

200-400

Mouse plasma

100-300

Rabbit serum

100-300

10% Rat liver tissue homogenate

200-400

10% Mouse kidney tissue homogenate

200-400

10% Rat brain tissue homogenate

200-400

10% Rat lung tissue homogenate

200-400

Note: The diluent is reagent 1.

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Drug and Chemical Toxicology (2020) IF: 2.405
    Dexamethasone increases renal free fatty acids and xanthine oxidase activity in female rats: could there be any gestational impact?

    DOI: 10.1080/01480545.2020.1784190

    PMID: 32597234

    Sample: Plasma

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