English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit

Cat:E-BC-F039 Citations (7)
Manual MSDS

Price: $ 450

Price: $ 300

Size:
96T 48T
Quantity:
  • Sample Type: serum;plasma;animal tissue;cells
  • Detection Instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)
  • Reasearch Areas: Metabolic Diseases;Glycolysis Lipid Metabolism
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Detection Principle Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.
Synonyms FFA;NEFA
Sample Type serum,plasma,animal tissue,cells
Sensitivity 0.58 μmol/L
Detection Range 0.58-20 μmol/L
Detection Method Fluorescence method
Assay Time 70 min
Precision inter-assay CV: 5.3% | intra-assay CV: 4.2%
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 10-20
Rat serum 200-400
Mouse plasma 100-300
Rabbit serum 100-300
10% Rat liver tissue homogenate 200-400
10% Mouse kidney tissue homogenate 200-400
10% Rat brain tissue homogenate 200-400
10% Rat lung tissue homogenate 200-400
The diluent is buffer solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.