Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit
Price: $ 450
Price: $ 300
Size:
96T
48T
Quantity:
- Sample Type: serum;plasma;animal tissue;cells
- Detection Instrument: Fluorescence microplate reader (Ex/Em=535 nm/590 nm)
- Reasearch Areas: Metabolic Diseases;Glycolysis Lipid Metabolism
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Product Details
Detection Principle | Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids. | ||||||||||||||||||
Synonyms | FFA;NEFA | ||||||||||||||||||
Sample Type | serum,plasma,animal tissue,cells | ||||||||||||||||||
Sensitivity | 0.58 μmol/L | ||||||||||||||||||
Detection Range | 0.58-20 μmol/L | ||||||||||||||||||
Detection Method | Fluorescence method | ||||||||||||||||||
Assay Time | 70 min | ||||||||||||||||||
Precision | inter-assay CV: 5.3% | intra-assay CV: 4.2% | ||||||||||||||||||
Storage | This product can be stored at -20°C for 12 months with shading light. | ||||||||||||||||||
Valid Period | 12 months | ||||||||||||||||||
Dilution Of Sample |
The recommended dilution factor for different samples is as follows (for reference only):
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The diluent is buffer solution. For the dilution of other sample types, please do pretest to confirm the dilution factor. |
Citations
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Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease
IF:17.694
Journal:Nature Communications
DOI:10.1038/s41467-022-35481-1
PMID:36572670
-
Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal
IF:8.755
Journal:CELL PROLIFERATION
-
Targeting hepatic sulfane sulfur/hydrogen sulfide signaling pathway with α-lipoic acid to prevent diabetes-induced liver injury via upregulating hepatic CSE/3-MST expression
IF:5.395
Journal:Diabetology & Metabolic Syndrome
DOI:10.1186/s13098-022-00921-x
PMID:36229864
-
Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway
IF:5.195
Journal:JOURNAL OF ETHNOPHARMACOLOGY
PMID:36283638
-
Association between duration of obesity and severity of ovarian dysfunction in rat-cafeteria diet approach
IF:4.49
Journal:JOURNAL OF NUTRITIONAL BIOCHEMISTRY
-
Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus
IF:4.223
Journal:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
DOI:10.1007/s11356-021-14871-w
PMID:34216342
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Dexamethasone increases renal free fatty acids and xanthine oxidase activity in female rats: could there be any gestational impact?
IF:2.405
Journal:DRUG AND CHEMICAL TOXICOLOGY
DOI:10.1080/01480545.2020.1784190
PMID:32597234