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γ-Aminobutyric Acid (GABA) Colorimetric Assay Kit

Cat.No.:E-BC-K852-M
Detection instrument: Microplate reader (630-650 nm, optimum wavelength: 640 nm)

Detection method: Colorimetric method

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96T
48T

Price: $260

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Protocols for metabolism assays

FAQ for metabolism assays

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For research use only. Order now, ship in 3 days

Detection principle

Phenol and sodium hypochlorite react with GABA to produce a blue-green product, which has maximum absorbance at 640 nm. GABA content can be calculated with the absorbance at 640 nm.

Performance characteristics

Sample type	plant and animal tissue
Sensitivity	0.06 μmol/mL
Detection range	0.06–10.0 μmol/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	40 min
Precision	Average inter-assay CV: 6.800%Average intra-assay CV: 4%
Other instruments required	Test tube, Micropipettor, Vortex mixer, Centrifuge, Water bath (95℃)
Storage	2-8℃
Valid period	12 months

Images

Exposure of 6PPD significantly increased the concentration of GABA in zebrafish brain. (P<0.05)

JW Ji et al investigate the eco-toxicity of 6PPD and its neurotoxicity. Concentration of γ-aminobutyric acid (GABA) of zebrafish brain was determined using GABA colorimetric assay kit (E-BC-K852-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.06–10.0 μmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Epipremnum aureum tissue homogenization	1
10% Green pepper tissue homogenization	1
10% Chinese yam tissue homogenization	1
10% Rat heart tissue homogenization	1
10% Rat liver tissue homogenization	1
10% Rat kidney tissue homogenization	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 7.963
Multiview behavior and neurotransmitter analysis of zebrafish dyskinesia induced by 6PPD and its metabolites

DOI: 10.1016/j.scitotenv.2022.156013

PMID: 35588826
Sample: serum
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (2022) IF: 4.114
Protective effect of low-dose radiation on doxorubicin-induced brain injury in mice

DOI: 10.1016/j.abb.2022.109390

PMID: 36067878
Sample: brain tissue
BRAIN RESEARCH BULLETIN (2019) IF: 3.103
Acetylcholine receptor agonist effect on seizure activity and GABAergic mechanisms involved in prolonged febrile seizure development in an animal model

DOI: 10.1016/j.brainresbull.2019.04.022

Sample: hippocampal tissue

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Reviews/Q&A

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Reviews
Q&A

O******** Submitted [ Nov 22 2023 ]

Asked: Can the kit be used to establish neuroprotective potential of medicinal plant in epilepsy study

adminSubmitted [ Nov 22 2023 ]

Answered: Hi Oguntunde, Thanks for your inquiry. E-BC-K852-M can detect plant samples. Our laboratory has verified that taro, potatoes, yams and other plant samples can be detected, but it has not verified medicinal plants. It is recommended that you buy a small-size kit to do a pre-test.

adminSubmitted [ Nov 22 2023 ]

Answered: The kit can detect plant samples. Our laboratory has verified that taro, potatoes, yams and other plant samples can be detected, but it has not verified medicinal plants. It is recommended that you buy a small-size kit to do a pre-test.

S*****rSubmitted [ Nov 17 2023 ]

Asked: Can I use this kit for GABA detection in iPSCs-derived neurons on a plate?

adminSubmitted [ Nov 17 2023 ]

Answered: Sorry, the γ-Aminobutyric Acid (GABA) Colorimetric Assay Kit can't detect iPSCs-derived neurons sample.

B***nSubmitted [ Nov 07 2023 ]

Asked: Hi, will this assay work for measuring the GABA concentration loaded in polymer-lipid nanoparticle formulations (it is in the form of an emulsion/colloid)?

adminSubmitted [ Nov 07 2023 ]

Answered: Hello Brian. Thanks for your message. Sorry, we haven't tested it with the sample, and it is recommended to do a pre-test. Theoretically it can be detected if the GABA content in the sample is above the sensitivity of the kit.

A*********tSubmitted [ Apr 04 2023 ]

Asked: Can this kit be used for GABA detection from bacterial culture>

adminSubmitted [ Apr 04 2023 ]

Answered: Unfortunately, this kit can not be used for bacterial.

D********eSubmitted [ Dec 04 2022 ]

Asked: γ-Aminobutyric Acid (GABA) Colorimetric Assay Kit Cat.No.:E-BC-K852-M Can it be used to evaluate GABA in rat brain tissue? How much tissue is needed per sample?

adminSubmitted [ Dec 04 2022 ]

Answered: This kit is suitable for GABA in rat brain tissue.The minimum sample size is 0.05g.

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