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γ-Glutamyl Transferase (GGT/γ-GT) Activity Assay Kit

Cat.No.:E-BC-K126-M
Detection instrument: Microplate reader(405 nm)

Detection method: Colorimetric method

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Detection principle

γ-GT catalyzes the transfer of gamma glutamyl group from glutamyl p-nitroaniline to N-glycyl glycine to produce p-nitroaniline, which has characteristic absorption peak at 405nm. The activity of γ-GT can be calculated according to the changing rate of absorbance value.

Performance characteristics

Synonyms	GGT/γ-GT
Sample type	Serum, plasma, animal tissue
Sensitivity	0.88 U/L
Detection range	0.88-399.4 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	20 min
Precision	Average inter-assay CV: 6.200%Average intra-assay CV: 4.200%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge, Water bath, Incubator
Storage	2-8℃
Valid period	12 months

Images

The activity of γ-GT was significantly increased in PBC patients. (**P<0.05)

GX Xu et al investigate the effect of endothelin receptor B in enhancing liver injury and pro-inflammatory. Activity of γ-glutamyl transferase of human serum was determined using γ-GT activity assay kit (E-BC-K126-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.88-399.4 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Mouse serum	1
Human serum	1
Rat serum	1
Dog serum	1
Human plasma	1
Horse serum	1
Porcine serum	1
Human hydrothorax	1
10% Mouse liver tissue homogenate	1
10% Mouse heart tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat lung tissue homogenate	1

Note: The diluent is reagent 3.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

PHYTOMEDICINE (2022) IF: 6.656
Carthami flos extract against carbon tetrachloride-induced liver fibrosis via alleviating angiogenesis in mice

DOI: 10.1016/j.phymed.2022.154517

PMID: 36332390
Sample: serum
Frontiers in Pharmacology (2021) IF: 5.811
Phillygenin Attenuates Carbon Tetrachloride-Induced Liver Fibrosis via Modulating Inflammation and Gut Microbiota

DOI: 10.3389/fphar.2021.756924

PMID: 34621179
Sample: serum
Scientific Reports (2022) IF: 4.996
Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

DOI: 10.1038/s41598-022-21816-x

Sample: serum
International Journal of Environmental Research and Public Health (2022) IF: 3.39
Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners

DOI: 10.3390/ijerph19010498

PMID: 35010758
Sample: plasma
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.65
San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress

DOI: 10.1155/2022/7832540

Sample: hepatic tissue,serum
Heliyon (2021)
Treatment with acetate during late pregnancy protects dams against testosterone-induced renal dysfunction

DOI: 10.1016/j.heliyon.2021.e05920

Sample: Renal

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