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Glutamate Synthase (GOGAT) Activity Assay Kit

  • Cat.No.:E-BC-K659-M

  • Detection instrument: Microplate reader (330-350 nm, optimum wavelength: 340 nm)

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Price: $380

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Detection principle

GOGAT catalysis the reaction that transfer the amino from glutamine to a-KG to form two molecules of glutamic acid using NADH as the electron donor. The decreasing rate of NADH that can be measured at 340 nm can reflect the activity of GOGAT.

Performance characteristics

Synonyms GOGAT
Sample type serum, plant and animal tissue
Sensitivity 8.84 U/L
Detection range 8.84–321.05 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 15 min
Precision Average inter-assay CV: 5.800%Average intra-assay CV: 2.500%
Other instruments required Centrifuge
Storage -20℃
Valid period 12 months


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